Start New Search

Search results are listed below.
Tip:
To quickly search the same gene in another data set, click the links from the last column. The locuslink number will be used as query.

 

No probe set gene Accession LocusLink Description Symbol CTRL FSK ACREB_FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC (ACREB_FSK vs FSK) FC (ACREB_FSK vs FSK) LFC 0hr 1hr_FSK 4hr_FSK (1hr_FSK vs 0hr) FC (1hr_FSK vs 0hr) LFC (4hr_FSK vs 0hr) FC (4hr_FSK vs 0hr) LFC notes Link
1 213898_at RNA binding motif protein 9 AW14937923543Consensus includes gb:AW149379 /FEA=EST /DB_XREF=gi:6197275 /DB_XREF=est:xf36d11.x1 /CLONE=IMAGE:2620149 /UG=Hs.5011 RNA binding motif protein 9 RBM9 92.55 82.14 84.62 -1.13 -0.7 1.03 0.55 92.53 104.84 93.05 1.13 0.81 1.01 0.76 "all, " Human_ChIP_chip Human_CRE_prediction Pancreatic_Islet_Expression
2 212104_s_at RNA binding motif protein 9 AL04974823543Consensus includes gb:N95026 /FEA=EST /DB_XREF=gi:1267308 /DB_XREF=est:zb45d12.s1 /CLONE=IMAGE:306551 /UG=Hs.5011 RNA binding motif protein 9 RBM9 501.79 367.82 473.5 -1.36 -0.94 1.29 1.01 86.31 104.86 95.5 1.21 0.3 1.11 0.31 "all, " Human_ChIP_chip Human_CRE_prediction Pancreatic_Islet_Expression
3 216215_s_at RNA binding motif protein 9 AL04974823543Consensus includes gb:AL049748 /DEF=Human DNA sequence from clone RP1-41P2 on chromosome 22 Contains the 3 part of the RBM9 gene for RNA binding motif protein 9 and the 3 part of the gene for a novel protein similar to part of APOL (apolipoprotein L) and TNF-inducible protein CG12... /FEA=mRNA_2 /DB_XREF=gi:6572235 /UG=Hs.5011 RNA binding motif protein 9 RBM9 517.38 428.15 330.77 -1.21 -1.07 -1.29 -1.12 158.1 218.39 145.34 1.38 0.92 -1.09 -0.8 "all, " Human_ChIP_chip Human_CRE_prediction Pancreatic_Islet_Expression
4 213901_x_at RNA binding motif protein 9 AW14937923543Consensus includes gb:AW149379 /FEA=EST /DB_XREF=gi:6197275 /DB_XREF=est:xf36d11.x1 /CLONE=IMAGE:2620149 /UG=Hs.5011 RNA binding motif protein 9 RBM9 327.87 146.07 238.8 -2.24 -1.72 1.63 1.16 64.29 58.14 74.83 -1.11 -0.47 1.16 0.56 "all, " Human_ChIP_chip Human_CRE_prediction Pancreatic_Islet_Expression

Sample Description:
Culture of human HEK293T cells are treated with cAMP agonist forskolin (FSK) for 1hr. Two control and two forskolin treated samples are collected, together with 2 forskolin treated samples from ACREB plasmid transfected cells. In addition to this data set, a different set of experiments showing FSK effect on 1hr and 4hr treatments is included. The two sets of data in HEK293T were generated at different times with different batch of cells, and comparison should be limited within each set. The cAMP induced genes at 1hr, however, was similar between the two sets. Total RNA was extracted using the Qiagen RNeasy kit.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133 Array Set HG-U133A array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK in control samples (LFC>=1) and this induction was inhibited by ACREB ((FSK_ACREB vs FSK) LFC<=-1), we consider it as a good camp induced gene and therefore a potential CREB target. In addition, induction by FSK with the second data set (time series) can further confirm the result from the first data set.