Search results are listed below.
Tip: To
quickly search the same gene in another data set, click the links from the last
column. The locuslink number will be used as query.
| No | probe set | gene | Accession | LocusLink | Description | Symbol | CTRL | FSK | ACREB_FSK | (FSK vs CTRL) FC | (FSK vs CTRL) LFC | (ACREB_FSK vs FSK) FC | (ACREB_FSK vs FSK) LFC | 0hr | 1hr_FSK | 4hr_FSK | (1hr_FSK vs 0hr) FC | (1hr_FSK vs 0hr) LFC | (4hr_FSK vs 0hr) FC | (4hr_FSK vs 0hr) LFC | notes | Link |
| 1 | 214313_s_at | translation initiation factor IF2 | BE138647 | 9669 | Consensus includes gb:BE138647 /FEA=EST /DB_XREF=gi:8601147 /DB_XREF=est:xr78h03.x2 /CLONE=IMAGE:2766293 /UG=Hs.158688 KIAA0741 gene product | IF2 | 12.19 | 59.75 | 23.39 | 4.9 | 2.01 | -2.55 | -1.12 | -41 | -55.54 | -44.75 | 1 | 0 | 1 | 0 | "all, " | Human_ChIP_chip Human_CRE_prediction Pancreatic_Islet_Expression |
| 2 | 201025_at | translation initiation factor IF2 | NM_015904.1 | 9669 | "Consensus includes gb:AB018284.1 /DEF=Homo sapiens mRNA for KIAA0741 protein, complete cds. /FEA=mRNA /GEN=KIAA0741 /PROD=KIAA0741 protein /DB_XREF=gi:3882202 /UG=Hs.158688 KIAA0741 gene product /FL=gb:AB018284.1 gb:AF078035.1 gb:NM_015904.1" | IF2 | 222.13 | 223.89 | 206.88 | 1.01 | 0.89 | -1.08 | -0.94 | 181.5 | 206.54 | 202.3 | 1.14 | 0.89 | 1.11 | 0.93 | "all, " | Human_ChIP_chip Human_CRE_prediction Pancreatic_Islet_Expression |
| 3 | 201026_at | translation initiation factor IF2 | NM_015904.1 | 9669 | "Consensus includes gb:AB018284.1 /DEF=Homo sapiens mRNA for KIAA0741 protein, complete cds. /FEA=mRNA /GEN=KIAA0741 /PROD=KIAA0741 protein /DB_XREF=gi:3882202 /UG=Hs.158688 KIAA0741 gene product /FL=gb:AB018284.1 gb:AF078035.1 gb:NM_015904.1" | IF2 | 104.42 | 109.2 | 115.15 | 1.05 | 0.82 | 1.05 | 0.8 | 118.51 | 103.98 | 94.66 | -1.14 | -0.76 | -1.25 | -0.88 | "all, " | Human_ChIP_chip Human_CRE_prediction Pancreatic_Islet_Expression |
| 4 | 201027_s_at | translation initiation factor IF2 | NM_015904.1 | 9669 | "gb:NM_015904.1 /DEF=Homo sapiens KIAA0741 gene product (IF2), mRNA. /FEA=mRNA /GEN=IF2 /PROD=translation initiation factor IF2 /DB_XREF=gi:7706231 /UG=Hs.158688 KIAA0741 gene product /FL=gb:AB018284.1 gb:AF078035.1 gb:NM_015904.1" | IF2 | 259.04 | 250.16 | 183.27 | -1.04 | -0.66 | -1.36 | -0.99 | 216.87 | 236.81 | 203.27 | 1.09 | 0.91 | -1.07 | -0.77 | "all, " | Human_ChIP_chip Human_CRE_prediction Pancreatic_Islet_Expression |
| 5 | 201024_x_at | translation initiation factor IF2 | NM_015904.1 | 9669 | Consensus includes gb:BG261322 /FEA=EST /DB_XREF=gi:12771138 /DB_XREF=est:602373079F1 /CLONE=IMAGE:4484563 /UG=Hs.158688 KIAA0741 gene product /FL=gb:AB018284.1 gb:AF078035.1 gb:NM_015904.1 | IF2 | 900.47 | 872.48 | 771.99 | -1.03 | -0.75 | -1.13 | -1.01 | 117.49 | 160.15 | 128.04 | 1.36 | 0.28 | 1.09 | 0.39 | "all, " | Human_ChIP_chip Human_CRE_prediction Pancreatic_Islet_Expression |
| 6 | 214314_s_at | translation initiation factor IF2 | BE138647 | 9669 | Consensus includes gb:BE138647 /FEA=EST /DB_XREF=gi:8601147 /DB_XREF=est:xr78h03.x2 /CLONE=IMAGE:2766293 /UG=Hs.158688 KIAA0741 gene product | IF2 | 657.72 | 569.71 | 575.33 | -1.15 | -0.86 | 1.01 | 0.92 | 109.14 | 166.51 | 141.36 | 1.53 | 0.38 | 1.3 | 1.11 | "all, " | Human_ChIP_chip Human_CRE_prediction Pancreatic_Islet_Expression |
Sample Description:
Culture of human HEK293T cells are treated with cAMP agonist forskolin (FSK)
for 1hr. Two control and two forskolin treated samples are collected, together
with 2 forskolin treated samples from ACREB plasmid transfected cells. In addition
to this data set, a different set of experiments showing FSK effect on 1hr and
4hr treatments is included. The two sets of data in HEK293T were generated at
different times with different batch of cells, and comparison should be limited
within each set. The cAMP induced genes at 1hr, however, was similar between
the two sets. Total RNA was extracted using the Qiagen RNeasy kit.
Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix
GeneChip Human Genome U133 Array Set HG-U133A array. Array data was analyzed
using the dChip software with PM-MM models.
Guidelines for Choosing camp Induced Genes in this Data
Set:
The fold change (FC) value is positive for increased expression and negative
for decrease expression. There is also a lower bound of fold change (LFC), which
is a conservative estimation of the fold change with 90% confidence. For this
data set, if a gene was induced by FSK in control samples (LFC>=1) and this
induction was inhibited by ACREB ((FSK_ACREB vs FSK) LFC<=-1), we consider
it as a good camp induced gene and therefore a potential CREB target. In addition,
induction by FSK with the second data set (time series) can further confirm
the result from the first data set.