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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 215533_s_at "ubiquitination factor E4B (UFD2 homolog, yeast)" AF09109310277UBE4B 686;E4;HDNB1;KIAA0684;UFD2 "Consensus includes gb:AF091093.1 /DEF=Homo sapiens clone 686 unknown mRNA, complete sequence. /FEA=mRNA /PROD=unknown /DB_XREF=gi:3860023 /UG=Hs.24594 ubiquitination factor E4B (homologous to yeast UFD2)" 182.79 200.93 1.1 0.95 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 202317_s_at "ubiquitination factor E4B (UFD2 homolog, yeast)" NM_00604810277UBE4B 686;E4;HDNB1;KIAA0684;UFD2 "gb:NM_006048.1 /DB_XREF=gi:5174482 /GEN=UBE4B /FEA=FLmRNA /CNT=190 /TID=Hs.24594.0 /TIER=FL /STK=0 /UG=Hs.24594 /LL=10277 /DEF=Homo sapiens ubiquitination factor E4B (homologous to yeast UFD2) (UBE4B), mRNA. /PROD=ubiquitination factor E4B (homologous to yeastUFD2) /FL=gb:AF043117.1 gb:NM_006048.1" 522.92 532.05 1.02 0.94 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 210685_s_at "ubiquitination factor E4B (UFD2 homolog, yeast)" AB02883910277UBE4B 686;E4;HDNB1;KIAA0684;UFD2 "gb:AB028839.1 /DEF=Homo sapiens HDNB1(homzygously deleted in neuroblastoma-1)UFD2 mRNA, complete cds. /FEA=mRNA /GEN=HDNB1UFD2 /PROD=homzygously deleted in neuroblastoma-1UFD2 /DB_XREF=gi:13516466 /UG=Hs.24594 ubiquitination factor E4B (homologous to yeast UFD2) /FL=gb:AB028839.1" 304.2 322.8 1.06 0.93 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 202316_x_at "ubiquitination factor E4B (UFD2 homolog, yeast)" NM_00604810277UBE4B 686;E4;HDNB1;KIAA0684;UFD2 Consensus includes gb:AW241715 /FEA=EST /DB_XREF=gi:6575469 /DB_XREF=est:xn74b08.x1 /CLONE=IMAGE:2700183 /UG=Hs.24594 ubiquitination factor E4B (homologous to yeast UFD2) /FL=gb:AF043117.1 gb:NM_006048.1 149.87 172.28 1.15 0.82 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.