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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 207359_at "calcium/calmodulin-dependent protein kinase kinase 2, beta" NM_00654910645CAMKK2 CAMKK;CAMKKB;KIAA0787;MGC15254 "gb:NM_006549.1 /DB_XREF=gi:5729894 /GEN=CAMKK2 /FEA=FLmRNA /CNT=5 /TID=Hs.108708.0 /TIER=FL /STK=2 /UG=Hs.108708 /LL=10645 /DEF=Homo sapiens calciumcalmodulin-dependent protein kinase kinase 2, beta (CAMKK2), mRNA. /PROD=calciumcalmodulin-dependent protein kinasekinase 2, beta /FL=gb:AF101264.1 gb:NM_006549.1" 209.33 244.25 1.17 1.02 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 213812_s_at "calcium/calmodulin-dependent protein kinase kinase 2, beta" AK02474810645CAMKK2 CAMKK;CAMKKB;KIAA0787;MGC15254 "gb:AK024748.1 /DB_XREF=gi:10437113 /FEA=mRNA /CNT=29 /TID=Hs.108708.2 /TIER=Stack /STK=16 /UG=Hs.108708 /LL=10645 /UG_GENE=CAMKK2 /UG_TITLE=calciumcalmodulin-dependent protein kinase kinase 2, beta /DEF=Homo sapiens cDNA: FLJ21095 fis, clone CAS03846." 1210.17 1222.84 1.01 0.93 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 212252_at "calcium/calmodulin-dependent protein kinase kinase 2, beta" AA18117910645CAMKK2 CAMKK;CAMKKB;KIAA0787;MGC15254 "gb:AA181179 /DB_XREF=gi:1764664 /DB_XREF=zp57b04.s1 /CLONE=IMAGE:624271 /FEA=mRNA /CNT=190 /TID=Hs.108708.3 /TIER=Stack /STK=16 /UG=Hs.108708 /LL=10645 /UG_GENE=CAMKK2 /UG_TITLE=calciumcalmodulin-dependent protein kinase kinase 2, beta" 276.91 287.76 1.04 0.91 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 210787_s_at "calcium/calmodulin-dependent protein kinase kinase 2, beta" AF14050710645CAMKK2 CAMKK;CAMKKB;KIAA0787;MGC15254 "gb:AF140507.1 /DB_XREF=gi:4877950 /GEN=CAMKKB /FEA=FLmRNA /CNT=2 /TID=Hs.108708.1 /TIER=FL /STK=0 /UG=Hs.108708 /LL=10645 /DEF=Homo sapiens Ca2+calmodulin-dependent protein kinase kinase beta (CAMKKB) mRNA, complete cds. /PROD=Ca2+calmodulin-dependent protein kinase kinasebeta /FL=gb:AF140507.1" 128.47 106.42 -1.21 -0.98 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.