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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 208325_s_at A kinase (PRKA) anchor protein 13 NM_00673811214AKAP13 AKAP-Lbc;BRX;FLJ11952;Ht31;LBC;PROTO-LB;PROTO-LBC;c-lbc "gb:NM_006738.1 /DB_XREF=gi:5803057 /GEN=LBC /FEA=FLmRNA /CNT=2 /TID=Hs.301946.0 /TIER=FL /STK=0 /UG=Hs.301946 /LL=3928 /DEF=Homo sapiens lymphoid blast crisis oncogene (LBC), mRNA. /PROD=lymphoid blast crisis oncogene /FL=gb:NM_006738.1 gb:U03634.1" 210.14 254.55 1.21 1 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 209535_s_at "gb:AF127481.1 /DB_XREF=gi:5199315 /GEN=proto-LBC /FEA=FLmRNA /CNT=157 /TID=Hs.301946.1 /TIER=FL /STK=0 /UG=Hs.301946 /LL=3928 /DEF=Homo sapiens non-ocogenic Rho GTPase-specific GTP exchange factor (proto-LBC) mRNA, complete cds. /PROD=non-ocogenic Rho GTPase-specific GTP exchangefactor /FL=gb:AF127481.1" AF12748111214AKAP13 AKAP-Lbc;BRX;FLJ11952;Ht31;LBC;PROTO-LB;PROTO-LBC;c-lbc "gb:AF127481.1 /DB_XREF=gi:5199315 /GEN=proto-LBC /FEA=FLmRNA /CNT=157 /TID=Hs.301946.1 /TIER=FL /STK=0 /UG=Hs.301946 /LL=3928 /DEF=Homo sapiens non-ocogenic Rho GTPase-specific GTP exchange factor (proto-LBC) mRNA, complete cds. /PROD=non-ocogenic Rho GTPase-specific GTP exchangefactor /FL=gb:AF127481.1" 251.91 253.8 1.01 0.91 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 222023_at A kinase (PRKA) anchor protein 13 AK02201411214AKAP13 AKAP-Lbc;BRX;FLJ11952;Ht31;LBC;PROTO-LB;PROTO-LBC;c-lbc "Consensus includes gb:AK022014.1 /DEF=Homo sapiens cDNA FLJ11952 fis, clone HEMBB1000831, weakly similar to Homo sapiens breast cancer nuclear receptor-binding auxiliary protein (BRX) mRNA. /FEA=mRNA /DB_XREF=gi:10433327 /UG=Hs.306619 Homo sapiens cDNA FLJ11952 fis, clone HEMBB1000831, weakly similar to Homo sapiens breast cancer nuclear receptor-binding auxiliary protein (BRX) mRNA" 19.09 32.67 1.71 0.38 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 213516_at "gb:AF126008.1 /DB_XREF=gi:4469557 /GEN=BRX /FEA=FLmRNA /CNT=38 /TID=Hs.301946.2 /TIER=FL /STK=0 /UG=Hs.301946 /LL=3928 /DEF=Homo sapiens breast cancer nuclear receptor-binding auxiliary protein (BRX) mRNA, complete cds. /PROD=breast cancer nuclear receptor-binding auxiliaryprotein /FL=gb:AF126008.1" AF12600811214AKAP13 AKAP-Lbc;BRX;FLJ11952;Ht31;LBC;PROTO-LB;PROTO-LBC;c-lbc "gb:AF126008.1 /DB_XREF=gi:4469557 /GEN=BRX /FEA=FLmRNA /CNT=38 /TID=Hs.301946.2 /TIER=FL /STK=0 /UG=Hs.301946 /LL=3928 /DEF=Homo sapiens breast cancer nuclear receptor-binding auxiliary protein (BRX) mRNA, complete cds. /PROD=breast cancer nuclear receptor-binding auxiliaryprotein /FL=gb:AF126008.1" -33.83 34.6 34.6 0.31 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
5 208324_at "gb:NM_006738.1 /DB_XREF=gi:5803057 /GEN=LBC /FEA=FLmRNA /CNT=2 /TID=Hs.301946.0 /TIER=FL /STK=0 /UG=Hs.301946 /LL=3928 /DEF=Homo sapiens lymphoid blast crisis oncogene (LBC), mRNA. /PROD=lymphoid blast crisis oncogene /FL=gb:NM_006738.1 gb:U03634.1" NM_00673811214AKAP13 AKAP-Lbc;BRX;FLJ11952;Ht31;LBC;PROTO-LB;PROTO-LBC;c-lbc "gb:NM_006738.1 /DB_XREF=gi:5803057 /GEN=LBC /FEA=FLmRNA /CNT=2 /TID=Hs.301946.0 /TIER=FL /STK=0 /UG=Hs.301946 /LL=3928 /DEF=Homo sapiens lymphoid blast crisis oncogene (LBC), mRNA. /PROD=lymphoid blast crisis oncogene /FL=gb:NM_006738.1 gb:U03634.1" 175.03 172.6 -1.01 -0.73 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
6 222024_s_at A kinase (PRKA) anchor protein 13 AK02201411214AKAP13 AKAP-Lbc;BRX;FLJ11952;Ht31;LBC;PROTO-LB;PROTO-LBC;c-lbc "Consensus includes gb:AK022014.1 /DEF=Homo sapiens cDNA FLJ11952 fis, clone HEMBB1000831, weakly similar to Homo sapiens breast cancer nuclear receptor-binding auxiliary protein (BRX) mRNA. /FEA=mRNA /DB_XREF=gi:10433327 /UG=Hs.306619 Homo sapiens cDNA FLJ11952 fis, clone HEMBB1000831, weakly similar to Homo sapiens breast cancer nuclear receptor-binding auxiliary protein (BRX) mRNA" 265.81 265.25 -1 -0.89 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
7 209534_x_at A kinase (PRKA) anchor protein 13 BF22282311214AKAP13 AKAP-Lbc;BRX;FLJ11952;Ht31;LBC;PROTO-LB;PROTO-LBC;c-lbc gb:BF222823 /DB_XREF=gi:11130000 /DB_XREF=7q23e12.x1 /CLONE=IMAGE:3699190 /FEA=FLmRNA /CNT=157 /TID=Hs.301946.1 /TIER=Stack /STK=21 /UG=Hs.301946 /LL=3928 /UG_GENE=LBC /UG_TITLE=lymphoid blast crisis oncogene /FL=gb:AF127481.1 716.34 653.51 -1.1 -0.92 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.