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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 204328_at epidermodysplasia verruciformis 1 NM_00726711322EVER1 EV1;EVIN1;LAK-4P;TMC6 "gb:NM_007267.2 /DB_XREF=gi:7305226 /GEN=LAK-4P /FEA=FLmRNA /CNT=77 /TID=Hs.16165.0 /TIER=FL /STK=0 /UG=Hs.16165 /LL=11322 /DEF=Homo sapiens expressed in activated TLAK lymphocytes (LAK-4P), mRNA. /PROD=expressed in activated TLAK lymphocytes /FL=gb:NM_007267.2 gb:AB002405.2" 313.8 321.2 1.02 0.91 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 214958_s_at epidermodysplasia verruciformis 1 AK02173811322EVER1 EV1;EVIN1;LAK-4P;TMC6 "gb:AK021738.1 /DB_XREF=gi:10432982 /FEA=mRNA /CNT=9 /TID=Hs.322422.0 /TIER=ConsEnd /STK=0 /UG=Hs.322422 /UG_TITLE=Homo sapiens cDNA FLJ11676 fis, clone HEMBA1004752, highly similar to Homo sapiens mRNA for LAK-4p /DEF=Homo sapiens cDNA FLJ11676 fis, clone HEMBA1004752, highly similar to Homo sapiens mRNA for LAK-4p." 306.94 324.05 1.06 0.91 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.