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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 208797_s_at golgin-67 AF20423123015GOLGIN-67 GOLGA5 Consensus includes gb:AI829170 /FEA=EST /DB_XREF=gi:5449841 /DB_XREF=est:wk76b03.x1 /CLONE=IMAGE:2421293 /UG=Hs.182982 golgin-67 /FL=gb:NM_015003.1 gb:AF204231.1 374.59 413.33 1.1 1.01 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 213212_x_at hypothetical protein LOC161527 AI63218123015GOLGIN-67 GOLGA5 gb:AI632181 /DB_XREF=gi:4683511 /DB_XREF=ts85h05.x1 /CLONE=IMAGE:2238105 /FEA=EST /CNT=62 /TID=Hs.182982.2 /TIER=Stack /STK=9 /UG=Hs.182982 /LL=23015 /UG_GENE=KIAA0855 /UG_TITLE=golgin-67 412.5 488.97 1.19 0.94 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 210424_s_at golgin-67 AF16462223015GOLGIN-67 GOLGA5 "gb:AF163441.1 /DEF=Homo sapiens golgin 67 mRNA, complete cds. /FEA=mRNA /PROD=golgin 67 /DB_XREF=gi:6969979 /UG=Hs.182982 golgin-67 /FL=gb:AF163441.1 gb:AF164622.1" 82.09 84.49 1.03 0.74 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 208798_x_at golgin-67 AF20423123015GOLGIN-67 GOLGA5 "gb:AF204231.1 /DB_XREF=gi:6808610 /GEN=GM88 /FEA=FLmRNA /CNT=326 /TID=Hs.182982.0 /TIER=FL+Stack /STK=93 /UG=Hs.182982 /LL=23015 /DEF=Homo sapiens 88-kDa Golgi protein (GM88) mRNA, complete cds. /PROD=88-kDa Golgi protein /FL=gb:NM_015003.1 gb:AF204231.1" 317.77 293.22 -1.08 -0.97 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
5 210425_x_at "gb:AF164622.1 /DB_XREF=gi:7211437 /GEN=GOLGA5 /FEA=FLmRNA /CNT=7 /TID=Hs.182982.1 /TIER=FL /STK=0 /UG=Hs.182982 /LL=23015 /DEF=Homo sapiens golgin-67 (GOLGA5) mRNA, complete cds. /PROD=golgin-67 /FL=gb:AF163441.1 gb:AF164622.1" AF16462223015GOLGIN-67 GOLGA5 "gb:AF164622.1 /DB_XREF=gi:7211437 /GEN=GOLGA5 /FEA=FLmRNA /CNT=7 /TID=Hs.182982.1 /TIER=FL /STK=0 /UG=Hs.182982 /LL=23015 /DEF=Homo sapiens golgin-67 (GOLGA5) mRNA, complete cds. /PROD=golgin-67 /FL=gb:AF163441.1 gb:AF164622.1" 340.71 284.67 -1.2 -1.02 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
6 213650_at golgin-67 AW00643823015GOLGIN-67 GOLGA5 Consensus includes gb:AW006438 /FEA=EST /DB_XREF=gi:5855216 /DB_XREF=est:wr16f06.x1 /CLONE=IMAGE:2481731 /UG=Hs.182982 golgin-67 140.84 107.94 -1.3 -1.06 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
7 213737_x_at hypothetical protein DKFZp434P162 AI62091123015GOLGIN-67 GOLGA5 gb:AI620911 /DB_XREF=gi:4630037 /DB_XREF=tu05d12.x1 /CLONE=IMAGE:2250167 /FEA=EST /CNT=25 /TID=Hs.205125.0 /TIER=Stack /STK=11 /UG=Hs.205125 /UG_TITLE=ESTs 652.97 550.2 -1.19 -1.11 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.