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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 217441_at "Consensus includes gb:AK023664.1 /DEF=Homo sapiens cDNA FLJ13602 fis, clone PLACE1010089, highly similar to Homo sapiens mRNA for KIAA1097 protein. /FEA=mRNA /DB_XREF=gi:10435655 /UG=Hs.173694 KIAA1097 protein" AK02366423032USP33 KIAA1097;VDU1 "Consensus includes gb:AK023664.1 /DEF=Homo sapiens cDNA FLJ13602 fis, clone PLACE1010089, highly similar to Homo sapiens mRNA for KIAA1097 protein. /FEA=mRNA /DB_XREF=gi:10435655 /UG=Hs.173694 KIAA1097 protein" 179.08 170.84 -1.05 -0.88 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 214843_s_at ubiquitin specific protease 33 AK02286423032USP33 KIAA1097;VDU1 "gb:AK022864.1 /DB_XREF=gi:10434503 /FEA=mRNA /CNT=13 /TID=Hs.173694.2 /TIER=ConsEnd /STK=0 /UG=Hs.173694 /LL=23032 /UG_GENE=KIAA1097 /UG_TITLE=KIAA1097 protein /DEF=Homo sapiens cDNA FLJ12802 fis, clone NT2RP2002124, weakly similar to UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 4 (EC 3.1.2.15)." 419.55 382.44 -1.1 -0.95 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 212513_s_at ubiquitin specific protease 33 AB02902023032USP33 KIAA1097;VDU1 "gb:AB029020.1 /DB_XREF=gi:5689530 /GEN=KIAA1097 /FEA=mRNA /CNT=117 /TID=Hs.173694.0 /TIER=Stack /STK=50 /UG=Hs.173694 /LL=23032 /DEF=Homo sapiens mRNA for KIAA1097 protein, partial cds. /PROD=KIAA1097 protein" 753.37 665.67 -1.13 -1.06 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.