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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 210806_at KIAA0998 AL13680823093KIAA0998  gb:AL136808.1 /DB_XREF=gi:12053126 /GEN=DKFZp434N2321 /FEA=FLmRNA /CNT=2 /TID=Hs.131525.1 /TIER=FL /STK=0 /UG=Hs.131525 /LL=23093 /DEF=Homo sapiens mRNA; cDNA DKFZp434N2321 (from clone DKFZp434N2321); complete cds. /PROD=hypothetical protein /FL=gb:AL136808.1 109.97 105.32 -1.04 -0.76 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 210539_at KIAA0998 AK02425923093KIAA0998  "gb:AK024259.1 /DB_XREF=gi:10436591 /FEA=FLmRNA /CNT=7 /TID=Hs.131525.0 /TIER=ConsEnd /STK=2 /UG=Hs.131525 /LL=23093 /UG_GENE=KIAA0998 /UG_TITLE=KIAA0998 protein /DEF=Homo sapiens cDNA FLJ14197 fis, clone NT2RP3002484, highly similar to Homo sapiens mRNA for KIAA0998 protein. /FL=gb:BC002766.1" 174.04 173 -1.01 -0.83 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 214672_at KIAA0998 AB02321523093KIAA0998  "Consensus includes gb:AB023215.1 /DEF=Homo sapiens mRNA for KIAA0998 protein, partial cds. /FEA=mRNA /GEN=KIAA0998 /PROD=KIAA0998 protein /DB_XREF=gi:4589639 /UG=Hs.131525 KIAA0998 protein" 317.91 304.47 -1.04 -0.97 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 208099_x_at KIAA0998 /// KIAA0998 NM_01507223093KIAA0998  "gb:NM_015072.1 /DEF=Homo sapiens KIAA0998 protein (KIAA0998), mRNA. /FEA=mRNA /GEN=KIAA0998 /PROD=KIAA0998 protein /DB_XREF=gi:13562091 /FL=gb:NM_015072.1" 133.38 108.68 -1.23 -1.06 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.