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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 210287_s_at fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) U011342321FLT1 FLT;VEGFR1 "gb:U01134.1 /DB_XREF=gi:451321 /GEN=sflt /FEA=FLmRNA /CNT=10 /TID=Hs.138671.1 /TIER=FL /STK=0 /UG=Hs.138671 /LL=2321 /DEF=Human soluble vascular endothelial cell growth factor receptor (sflt) mRNA, complete cds. /PROD=soluble vascular endothelial cell growth factorreceptor /FL=gb:U01134.1" 113.98 169.1 1.48 1.28 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 222033_s_at Consensus includes gb:AA058828 /FEA=EST /DB_XREF=gi:1551654 /DB_XREF=est:zf66f10.s1 /CLONE=IMAGE:381931 /UG=Hs.138671 fms-related tyrosine kinase 1 (vascular endothelial growth factorvascular permeability factor receptor) AA0588282321FLT1 FLT;VEGFR1 Consensus includes gb:AA058828 /FEA=EST /DB_XREF=gi:1551654 /DB_XREF=est:zf66f10.s1 /CLONE=IMAGE:381931 /UG=Hs.138671 fms-related tyrosine kinase 1 (vascular endothelial growth factorvascular permeability factor receptor) 509.37 572 1.12 0.97 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 204406_at "gb:NM_002019.1 /DB_XREF=gi:4503748 /GEN=FLT1 /FEA=FLmRNA /CNT=80 /TID=Hs.138671.0 /TIER=FL /STK=1 /UG=Hs.138671 /LL=2321 /DEF=Homo sapiens fms-related tyrosine kinase 1 (vascular endothelial growth factorvascular permeability factor receptor) (FLT1), mRNA. /PROD=fms-related tyrosine kinase 1 (vascularendothelial growth factorvascular permeability factorreceptor) /FL=gb:NM_002019.1 gb:AF063657.1" NM_0020192321FLT1 FLT;VEGFR1 "gb:NM_002019.1 /DB_XREF=gi:4503748 /GEN=FLT1 /FEA=FLmRNA /CNT=80 /TID=Hs.138671.0 /TIER=FL /STK=1 /UG=Hs.138671 /LL=2321 /DEF=Homo sapiens fms-related tyrosine kinase 1 (vascular endothelial growth factorvascular permeability factor receptor) (FLT1), mRNA. /PROD=fms-related tyrosine kinase 1 (vascularendothelial growth factorvascular permeability factorreceptor) /FL=gb:NM_002019.1 gb:AF063657.1" 68.43 58.65 -1.17 -0.72 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.