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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 213092_x_at "DnaJ (Hsp40) homolog, subfamily C, member 9" AW24177923234DNAJC9 JDD1;KIAA0974;SB73 gb:AW241779 /DB_XREF=gi:6575533 /DB_XREF=xn75a03.x1 /CLONE=IMAGE:2700268 /FEA=EST /CNT=82 /TID=Hs.44131.2 /TIER=Stack /STK=8 /UG=Hs.44131 /LL=23234 /UG_GENE=KIAA0974 /UG_TITLE=KIAA0974 protein 105.28 127.9 1.21 0.85 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 213896_x_at KIAA0974 BE85654923234DNAJC9 JDD1;KIAA0974;SB73 gb:BE856549 /DB_XREF=gi:10369681 /DB_XREF=7f64b02.x1 /CLONE=IMAGE:3299403 /FEA=EST /CNT=25 /TID=Hs.44131.0 /TIER=Stack /STK=14 /UG=Hs.44131 /LL=23234 /UG_GENE=KIAA0974 /UG_TITLE=KIAA0974 protein 123.7 114.32 -1.08 -0.84 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 213463_s_at KIAA0974 AW30050423234DNAJC9 JDD1;KIAA0974;SB73 gb:AW300504 /DB_XREF=gi:6710181 /DB_XREF=xs66c11.x1 /CLONE=IMAGE:2774612 /FEA=mRNA /CNT=45 /TID=Hs.44131.1 /TIER=Stack /STK=15 /UG=Hs.44131 /LL=23234 /UG_GENE=KIAA0974 /UG_TITLE=KIAA0974 protein 344.36 316.88 -1.09 -0.98 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 213088_s_at "DnaJ (Hsp40) homolog, subfamily C, member 9" BE55134023234DNAJC9 JDD1;KIAA0974;SB73 Consensus includes gb:BE551340 /FEA=EST /DB_XREF=gi:9793032 /DB_XREF=est:7b63c12.x1 /CLONE=IMAGE:3232918 /UG=Hs.44131 KIAA0974 protein 577.34 502.98 -1.15 -1.04 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
5 213458_at KIAA0974 AB02319123234DNAJC9 JDD1;KIAA0974;SB73 "gb:AB023191.1 /DB_XREF=gi:4589591 /GEN=KIAA0974 /FEA=mRNA /CNT=45 /TID=Hs.44131.1 /TIER=ConsEnd /STK=0 /UG=Hs.44131 /LL=23234 /DEF=Homo sapiens mRNA for KIAA0974 protein, partial cds. /PROD=KIAA0974 protein" 132.61 93.76 -1.41 -1.04 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.