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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 213983_s_at SCC-112 protein AW99121923244KIAA0648 SCC-112 Consensus includes gb:AW991219 /FEA=EST /DB_XREF=gi:8249392 /DB_XREF=est:RDEV128 /UG=Hs.31921 KIAA0648 protein 202.38 234.47 1.16 0.99 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 213984_at SCC-112 protein AW99121923244KIAA0648 SCC-112 gb:AW991219 /DB_XREF=gi:8249392 /DB_XREF=RDEV128 /FEA=EST /CNT=21 /TID=Hs.31921.0 /TIER=Stack /STK=12 /UG=Hs.31921 /LL=23244 /UG_GENE=KIAA0648 /UG_TITLE=KIAA0648 protein 173.93 195.45 1.12 0.98 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 212140_at SCC-112 protein AB01454823244KIAA0648 SCC-112 "gb:AB014548.1 /DB_XREF=gi:3327109 /GEN=KIAA0648 /FEA=mRNA /CNT=222 /TID=Hs.31921.1 /TIER=Stack /STK=68 /UG=Hs.31921 /LL=23244 /DEF=Homo sapiens mRNA for KIAA0648 protein, partial cds. /PROD=KIAA0648 protein" 500.85 518.91 1.04 0.94 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 212138_at SCC-112 protein AB01454823244KIAA0648 SCC-112 "Consensus includes gb:AK021757.1 /DEF=Homo sapiens cDNA FLJ11695 fis, clone HEMBA1005019, highly similar to Homo sapiens mRNA for KIAA0648 protein. /FEA=mRNA /DB_XREF=gi:10433003 /UG=Hs.31921 KIAA0648 protein" 763.18 733.41 -1.04 -0.94 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.