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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 210801_at putative dimethyladenosine transferase BC00284127292HSA9761  "gb:BC002841.1 /DB_XREF=gi:12803982 /FEA=FLmRNA /CNT=2 /TID=Hs.125819.1 /TIER=FL /STK=0 /UG=Hs.125819 /LL=27292 /UG_GENE=HSA9761 /DEF=Homo sapiens, Similar to putative dimethyladenosine transferase, clone MGC:3382, mRNA, complete cds. /PROD=Similar to putative dimethyladenosinetransferase /FL=gb:BC002841.1" 34.58 35.16 1.02 0.59 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 210802_s_at putative dimethyladenosine transferase BC00284127292HSA9761  "gb:BC002841.1 /DB_XREF=gi:12803982 /FEA=FLmRNA /CNT=2 /TID=Hs.125819.1 /TIER=FL /STK=0 /UG=Hs.125819 /LL=27292 /UG_GENE=HSA9761 /DEF=Homo sapiens, Similar to putative dimethyladenosine transferase, clone MGC:3382, mRNA, complete cds. /PROD=Similar to putative dimethyladenosinetransferase /FL=gb:BC002841.1" 242.6 240.93 -1.01 -0.89 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 213598_at gb:W87688 /DB_XREF=gi:1401813 /DB_XREF=zh68c03.s1 /CLONE=IMAGE:417220 /FEA=EST /CNT=31 /TID=Hs.125819.2 /TIER=Stack /STK=18 /UG=Hs.125819 /LL=27292 /UG_GENE=HSA9761 /UG_TITLE=putative dimethyladenosine transferase W8768827292HSA9761  gb:W87688 /DB_XREF=gi:1401813 /DB_XREF=zh68c03.s1 /CLONE=IMAGE:417220 /FEA=EST /CNT=31 /TID=Hs.125819.2 /TIER=Stack /STK=18 /UG=Hs.125819 /LL=27292 /UG_GENE=HSA9761 /UG_TITLE=putative dimethyladenosine transferase 212.07 193.88 -1.09 -0.93 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 217106_x_at putative dimethyladenosine transferase AF09107827292HSA9761  "Consensus includes gb:AF091078.1 /DEF=Homo sapiens clone 559 unknown mRNA, complete sequence. /FEA=mRNA /PROD=unknown /DB_XREF=gi:3859993 /UG=Hs.125819 putative dimethyladenosine transferase" 575.19 556.17 -1.03 -0.94 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
5 204405_x_at putative dimethyladenosine transferase NM_01447327292HSA9761  "gb:NM_014473.1 /DB_XREF=gi:7657197 /GEN=HSA9761 /FEA=FLmRNA /CNT=58 /TID=Hs.125819.0 /TIER=FL+Stack /STK=15 /UG=Hs.125819 /LL=27292 /DEF=Homo sapiens putative dimethyladenosine transferase (HSA9761), mRNA. /PROD=putative dimethyladenosine transferase /FL=gb:AF102147.1 gb:NM_014473.1" 573.41 561.29 -1.02 -0.96 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.