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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 211327_x_at hemochromatosis AF1498043077HFE HFE1;HH;HLA-H "gb:AF149804.1 /DEF=Homo sapiens hemochromatosis protein splice variant 562-878del (HFE) mRNA, complete cds. /FEA=mRNA /GEN=HFE /PROD=hemochromatosis protein splice variant562-878del /DB_XREF=gi:11093523 /UG=Hs.20019 hemochromatosis /FL=gb:AF149804.1" 335.39 465.73 1.39 1.23 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 206087_x_at hemochromatosis NM_0004103077HFE HFE1;HH;HLA-H "gb:NM_000410.1 /DB_XREF=gi:4504376 /GEN=HFE /FEA=FLmRNA /CNT=24 /TID=Hs.20019.0 /TIER=FL+Stack /STK=11 /UG=Hs.20019 /LL=3077 /DEF=Homo sapiens hemochromatosis (HFE), mRNA. /PROD=hemochromatosis /FL=gb:NM_000410.1 gb:U60319.1 gb:AF115265.1" 631.14 645.7 1.02 0.95 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 211330_s_at hemochromatosis AF1442423077HFE HFE1;HH;HLA-H "gb:AF144242.1 /DB_XREF=gi:11094324 /GEN=HFE /FEA=FLmRNA /CNT=1 /TID=Hs.20019.5 /TIER=FL /STK=0 /UG=Hs.20019 /LL=3077 /DEF=Homo sapiens hemochromatosis splice variant delE3 mRNA, complete cds. /PROD=hemochromatosis splice variant delE3 /FL=gb:AF144242.1" 403.4 413.22 1.02 0.91 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 211326_x_at hemochromatosis AF1506643077HFE HFE1;HH;HLA-H "gb:AF150664.1 /DEF=Homo sapiens hemochromatosis splice variant delE3,intron3ins,intron4ins (HFE) mRNA, complete cds. /FEA=mRNA /GEN=HFE /PROD=hemochromatosis splice variantdelE3,intron3ins,intron4ins /DB_XREF=gi:11093525 /UG=Hs.20019 hemochromatosis /FL=gb:AF150664.1" 125.72 126.59 1.01 0.63 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
5 211866_x_at hemochromatosis AF0794093077HFE HFE1;HH;HLA-H "gb:AF079409.1 /DEF=Homo sapiens Hemochromatosis splice variant delE2(14E4) (HFE) mRNA, complete cds. /FEA=CDS /GEN=HFE /PROD=hemochromatosis splice variant delE214E4 /DB_XREF=gi:3695110 /UG=Hs.20019 hemochromatosis /FL=gb:AF079409.1" -240.85 291.16 291.16 0.23 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
6 206086_x_at hemochromatosis NM_0004103077HFE HFE1;HH;HLA-H "gb:AF115265.1 /DEF=Homo sapiens hemochromatosis termination variant terE6 (HFE) mRNA, complete cds. /FEA=mRNA /GEN=HFE /PROD=hemochromatosis termination variant terE6 /DB_XREF=gi:11094314 /UG=Hs.20019 hemochromatosis /FL=gb:AF115265.1 gb:U60319.1 gb:NM_000410.1" 189.56 139.48 -1.36 -0.83 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
7 211863_x_at hemochromatosis AF0794083077HFE HFE1;HH;HLA-H "gb:AF079408.1 /DB_XREF=gi:3695108 /GEN=HFE /FEA=FLmRNA /CNT=1 /TID=Hs.20019.10 /TIER=FL /STK=0 /UG=Hs.20019 /LL=3077 /DEF=Homo sapiens hemochromatosis splice variant delE2 (HFE) mRNA, complete cds. /PROD=hemochromatosis splice variant delE2 /FL=gb:AF079408.1" 314.13 308.49 -1.02 -0.84 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
8 211328_x_at hemochromatosis AF1442443077HFE HFE1;HH;HLA-H "gb:AF144244.1 /DB_XREF=gi:11094328 /GEN=HFE /FEA=FLmRNA /CNT=2 /TID=Hs.20019.3 /TIER=FL /STK=0 /UG=Hs.20019 /LL=3077 /DEF=Homo sapiens hemochromatosis splice variant 388-2283del,intron6ins mRNA, complete cds. /PROD=hemochromatosis splice variant388-2283del,intron6ins /FL=gb:AF144244.1 gb:AF144245.1" 856.41 765.94 -1.12 -0.9 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
9 214647_s_at hemochromatosis BG4024603077HFE HFE1;HH;HLA-H Consensus includes gb:BG402460 /FEA=EST /DB_XREF=gi:13295908 /DB_XREF=est:602466163F1 /CLONE=IMAGE:4594359 /UG=Hs.20019 hemochromatosis /FL=gb:AF079407.1 42.45 26.82 -1.58 -0.98 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
10 211331_x_at hemochromatosis AF1442433077HFE HFE1;HH;HLA-H "gb:AF144243.1 /DB_XREF=gi:11094326 /GEN=HFE /FEA=FLmRNA /CNT=1 /TID=Hs.20019.6 /TIER=FL /STK=0 /UG=Hs.20019 /LL=3077 /DEF=Homo sapiens hemochromatosis splice variant 495-2314del mRNA, complete cds. /PROD=hemochromatosis splice variant 495-2314del /FL=gb:AF144243.1" 783.62 693.32 -1.13 -0.99 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
11 210864_x_at hemochromatosis AF1442403077HFE HFE1;HH;HLA-H "gb:AF144240.1 /DB_XREF=gi:11094320 /GEN=HFE /FEA=FLmRNA /CNT=2 /TID=Hs.20019.7 /TIER=FL /STK=0 /UG=Hs.20019 /LL=3077 /DEF=Homo sapiens hemochromatosis splice variant 1043-2283del,intron6ins mRNA, complete cds. /PROD=hemochromatosis splice variant1043-2283del,intron6ins /FL=gb:AF144240.1" 435.68 389.18 -1.12 -1.04 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
12 211332_x_at hemochromatosis AF1442413077HFE HFE1;HH;HLA-H "gb:AF144241.1 /DEF=Homo sapiens hemochromatosis splice variant 861-2305del mRNA, complete cds. /FEA=mRNA /GEN=HFE /PROD=hemochromatosis splice variant 861-2305del /DB_XREF=gi:11094322 /UG=Hs.20019 hemochromatosis /FL=gb:AF144239.1 gb:AF144241.1" 661.02 567.91 -1.16 -1.08 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
13 211329_x_at hemochromatosis AF1152643077HFE HFE1;HH;HLA-H "gb:AF115264.1 /DEF=Homo sapiens hemochromatosis splice variant delE3-7 (HFE) mRNA, complete cds. /FEA=mRNA /GEN=HFE /PROD=hemochromatosis splice variant delE3-7 /DB_XREF=gi:11094312 /UG=Hs.20019 hemochromatosis /FL=gb:AF115264.1" 1121.13 969.1 -1.16 -1.1 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.