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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 208687_x_at heat shock 70kDa protein 8 AF3528323312HSPA8 HSC54;HSC70;HSC71;HSP71;HSP73;HSPA10;LAP1;MGC29929 "gb:AF352832.1 /DB_XREF=gi:13273303 /GEN=HSC70 /FEA=FLmRNA /CNT=479 /TID=Hs.180414.0 /TIER=FL /STK=0 /UG=Hs.180414 /LL=3312 /DEF=Homo sapiens constitutive heat shock protein 70 (HSC70) mRNA, complete cds. /PROD=constitutive heat shock protein 70 /FL=gb:AF352832.1 gb:NM_006597.1" 3958.2 4185.3 1.06 0.99 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 210338_s_at heat shock 70kDa protein 8 AB0349513312HSPA8 HSC54;HSC70;HSC71;HSP71;HSP73;HSPA10;LAP1;MGC29929 "gb:AB034951.1 /DB_XREF=gi:11526572 /GEN=HSC54 /FEA=FLmRNA /CNT=11 /TID=Hs.180414.2 /TIER=FL /STK=0 /UG=Hs.180414 /LL=3312 /DEF=Homo sapiens HSC54 mRNA for heat shock cognate protein 54, complete cds. /PROD=heat shock cognate protein 54 /FL=gb:AB034951.1" 4232.73 4414.07 1.04 0.99 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 221891_x_at heat shock 70kDa protein 8 AA7040043312HSPA8 HSC54;HSC70;HSC71;HSP71;HSP73;HSPA10;LAP1;MGC29929 Consensus includes gb:AA704004 /FEA=EST /DB_XREF=gi:2713922 /DB_XREF=est:ag46h11.s1 /CLONE=IMAGE:1119909 /UG=Hs.180414 heat shock 70kD protein 8 5415.96 5731.08 1.06 0.99 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.