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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 204628_s_at "integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)" NM_0002123690ITGB3 CD61;GP3A;GPIIIa "gb:NM_000212.1 /DB_XREF=gi:4557676 /GEN=ITGB3 /FEA=FLmRNA /CNT=66 /TID=Hs.87149.0 /TIER=FL /STK=0 /UG=Hs.87149 /LL=3690 /DEF=Homo sapiens integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) (ITGB3), mRNA. /PROD=integrin beta chain, beta 3 precursor /FL=gb:J02703.1 gb:M35999.1 gb:M20311.1 gb:NM_000212.1" 217.69 254.73 1.17 1.01 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 204625_s_at "integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)" BF1156583690ITGB3 CD61;GP3A;GPIIIa "gb:BF115658 /DB_XREF=gi:10985134 /DB_XREF=7n82b10.x1 /CLONE=IMAGE:3571146 /FEA=FLmRNA /CNT=66 /TID=Hs.87149.0 /TIER=Stack /STK=16 /UG=Hs.87149 /LL=3690 /UG_GENE=ITGB3 /UG_TITLE=integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) /FL=gb:J02703.1 gb:M35999.1 gb:M20311.1 gb:NM_000212.1" 361.3 366.37 1.01 0.94 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 216261_at "integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)" AI1514793690ITGB3 CD61;GP3A;GPIIIa "Consensus includes gb:AI151479 /FEA=EST /DB_XREF=gi:3679948 /DB_XREF=est:qc74g06.x1 /CLONE=IMAGE:1715386 /UG=Hs.87149 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)" 183.78 186.14 1.01 0.87 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 211579_at "integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)" U952043690ITGB3 CD61;GP3A;GPIIIa "gb:U95204.1 /DB_XREF=gi:2443451 /FEA=FLmRNA /CNT=1 /TID=Hs.87149.1 /TIER=FL /STK=0 /UG=Hs.87149 /LL=3690 /UG_GENE=ITGB3 /DEF=Homo sapiens platelet membrane glycoprotein IIIa beta subunit mRNA, complete cds. /PROD=platelet membrane glycoprotein IIIa betasubunit /FL=gb:U95204.1" 321.21 304.33 -1.06 -0.88 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
5 204627_s_at "integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)" NM_0002123690ITGB3 CD61;GP3A;GPIIIa "gb:M35999.1 /DEF=Human platelet glycoprotein IIIa (GPIIIa) mRNA, complete cds. /FEA=mRNA /DB_XREF=gi:183532 /UG=Hs.87149 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) /FL=gb:J02703.1 gb:M35999.1 gb:M20311.1 gb:NM_000212.1" 273.18 267.49 -1.02 -0.91 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
6 204626_s_at "integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)" NM_0002123690ITGB3 CD61;GP3A;GPIIIa "gb:J02703.1 /DEF=Human endothelial membrane glycoprotein IIIa (GPIIIa) mRNA, complete cds. /FEA=mRNA /GEN=ITGB3 /DB_XREF=gi:183452 /UG=Hs.87149 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) /FL=gb:J02703.1 gb:M35999.1 gb:M20311.1 gb:NM_000212.1" 1403.01 1241.35 -1.13 -1 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
7 215240_at "integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)" AI1898393690ITGB3 CD61;GP3A;GPIIIa "gb:AI189839 /DB_XREF=gi:3741048 /DB_XREF=qd23e12.x1 /CLONE=IMAGE:1724590 /FEA=mRNA /CNT=5 /TID=Hs.87149.2 /TIER=ConsEnd /STK=2 /UG=Hs.87149 /LL=3690 /UG_GENE=ITGB3 /UG_TITLE=integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)" 302.71 249.06 -1.22 -1.13 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.