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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 207796_x_at "killer cell lectin-like receptor subfamily D, member 1" NM_0073343824KLRD1 CD94 "gb:NM_007334.1 /DB_XREF=gi:7669498 /GEN=KLRD1 /FEA=FLmRNA /CNT=3 /TID=Hs.41682.0 /TIER=FL /STK=0 /UG=Hs.41682 /LL=3824 /DEF=Homo sapiens killer cell lectin-like receptor subfamily D, member 1 (KLRD1), transcript variant 2, mRNA. /PROD=killer cell lectin-like receptor subfamily D,member 1 isoform 2 /FL=gb:AB009597.1 gb:NM_007334.1" 89.05 69.16 -1.29 -0.79 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 207795_s_at "killer cell lectin-like receptor subfamily D, member 1" AB0095973824KLRD1 CD94 "gb:AB009597.1 /DEF=Homo sapiens mRNA for CD94, complete cds. /FEA=mRNA /GEN=CD94 alt /PROD=CD94 /DB_XREF=gi:2804283 /UG=Hs.41682 killer cell lectin-like receptor subfamily D, member 1 /FL=gb:AB009597.1 gb:NM_007334.1" 189.56 178.83 -1.06 -0.89 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 210606_x_at "killer cell lectin-like receptor subfamily D, member 1" U306103824KLRD1 CD94 "gb:U30610.1 /DEF=Human CD94 protein mRNA, complete cds. /FEA=mRNA /PROD=CD94 protein /DB_XREF=gi:1098616 /UG=Hs.41682 killer cell lectin-like receptor subfamily D, member 1 /FL=gb:U30610.1 gb:NM_002262.2" 224.68 198.43 -1.13 -0.94 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.