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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 207013_s_at matrix metalloproteinase 16 (membrane-inserted) AB0093034325MMP16 MMP-X2;MT-MMP2;MT-MMP3;MT3-MMP "gb:AB009303.1 /DB_XREF=gi:2662305 /FEA=FLmRNA /CNT=23 /TID=Hs.90800.1 /TIER=FL /STK=0 /UG=Hs.90800 /LL=4325 /UG_GENE=MMP16 /DEF=Homo sapiens mRNA for membrane-type matrix metalloproteinase 3, complete cds. /PROD=membrane-type matrix metalloproteinase 3 /FL=gb:AB009303.1 gb:D83646.1 gb:D85511.1 gb:NM_005941.2" 569.19 680.86 1.2 1.07 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 208166_at matrix metalloproteinase 16 (membrane-inserted) NM_0225644325MMP16 MMP-X2;MT-MMP2;MT-MMP3;MT3-MMP "gb:NM_022564.1 /DB_XREF=gi:13027799 /GEN=MMP16 /FEA=FLmRNA /CNT=2 /TID=Hs.90800.0 /TIER=FL /STK=0 /UG=Hs.90800 /LL=4325 /DEF=Homo sapiens matrix metalloproteinase 16 (membrane-inserted) (MMP16), transcript variant 2, mRNA. /PROD=matrix metalloproteinase 16, isoform 2 /FL=gb:D83647.1 gb:NM_022564.1" 38.9 57.64 1.48 0.91 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 207012_at matrix metalloproteinase 16 (membrane-inserted) AB0093034325MMP16 MMP-X2;MT-MMP2;MT-MMP3;MT3-MMP Consensus includes gb:U79292.1 /DEF=Human clone 23734 mRNA sequence. /FEA=mRNA /DB_XREF=gi:1710273 /UG=Hs.90800 matrix metalloproteinase 16 (membrane-inserted) /FL=gb:D83646.1 gb:NM_005941.2 gb:D85511.1 gb:AB009303.1 266.32 275.01 1.03 0.88 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 208167_s_at matrix metalloproteinase 16 (membrane-inserted) NM_0225644325MMP16 MMP-X2;MT-MMP2;MT-MMP3;MT3-MMP "gb:NM_022564.1 /DB_XREF=gi:13027799 /GEN=MMP16 /FEA=FLmRNA /CNT=2 /TID=Hs.90800.0 /TIER=FL /STK=0 /UG=Hs.90800 /LL=4325 /DEF=Homo sapiens matrix metalloproteinase 16 (membrane-inserted) (MMP16), transcript variant 2, mRNA. /PROD=matrix metalloproteinase 16, isoform 2 /FL=gb:D83647.1 gb:NM_022564.1" 76.32 52.67 -1.45 -0.97 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.