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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 209636_at nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) BC0028444791NFKB2 LYT-10;LYT10 "gb:BC002844.1 /DEF=Homo sapiens, Similar to nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, p49p100, clone MGC:3685, mRNA, complete cds. /FEA=mRNA /PROD=Similar to nuclear factor of kappa lightpolypeptide gene enhancer in B-cells 2, p49p100 /DB_XREF=gi:12803988 /UG=Hs.73090 nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49p100) /FL=gb:BC002844.1" 163.4 213.22 1.3 1.14 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 211524_at nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) U096094791NFKB2 LYT-10;LYT10 "gb:U09609.1 /DEF=Human p80HT (p80HTNKFB-2) mRNA, complete cds. /FEA=mRNA /GEN=p80HTNKFB-2 /PROD=p80HT /DB_XREF=gi:495193 /UG=Hs.73090 nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49p100) /FL=gb:U09609.1" 171.75 172.7 1.01 0.77 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 207535_s_at nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) NM_0025024791NFKB2 LYT-10;LYT10 "gb:NM_002502.1 /DB_XREF=gi:4505382 /GEN=NFKB2 /FEA=FLmRNA /CNT=8 /TID=Hs.73090.0 /TIER=FL /STK=0 /UG=Hs.73090 /LL=4791 /DEF=Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49p100) (NFKB2), mRNA. /PROD=nuclear factor of kappa light polypeptide geneenhancer in B-cells 2 (p49p100) /FL=gb:NM_002502.1" 369.79 342.05 -1.08 -0.99 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.