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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 214563_at "protocadherin gamma subfamily C, 3" AF1525245098PCDHGC3 PC43;PCDH-GAMMA-C3;PCDH2 "gb:AF152524.1 /DB_XREF=gi:5457095 /GEN=PCDH-gamma-C3 /FEA=FLmRNA /CNT=2 /TID=Hs.173310.1 /TIER=FL /STK=0 /UG=Hs.173310 /LL=5098 /DEF=Homo sapiens protocadherin gamma C3 short form protein (PCDH-gamma-C3) variable region sequence, complete cds. /PROD=protocadherin gamma C3 short form protein /FL=gb:AF152524.1" 374.66 395.53 1.06 0.93 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 214564_s_at "protocadherin gamma subfamily C, 3" AF1525245098PCDHGC3 PC43;PCDH-GAMMA-C3;PCDH2 "Consensus includes gb:AF152524.1 /DEF=Homo sapiens protocadherin gamma C3 short form protein (PCDH-gamma-C3) variable region sequence, complete cds. /FEA=CDS /GEN=PCDH-gamma-C3 /PROD=protocadherin gamma C3 short form protein /DB_XREF=gi:5457095 /UG=Hs.173310 protocadherin gamma subfamily C, 3 /FL=gb:AF152524.1" 115.4 109.68 -1.05 -0.88 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 205717_x_at "protocadherin gamma subfamily A, 1 /// protocadherin gamma subfamily A, 10 /// protocadherin gamma subfamily A, 11 /// protocadherin gamma subfamily A, 12 /// protocadherin gamma subfamily A, 2 /// protocadherin gamma subfamily A, 3 /// protocadherin gamma subfamily A, 4 /// protocadherin gamma subfamily A, 5 /// protocadherin gamma subfamily A, 6 /// protocadherin gamma subfamily A, 7 /// protocadherin gamma subfamily A, 8 /// protocadherin gamma subfamily A, 9 /// protocadherin gamma subfamily B, 1 /// protocadherin gamma subfamily B, 2 /// protocadherin gamma subfamily B, 3 /// protocadherin gamma subfamily B, 4 /// protocadherin gamma subfamily B, 5 /// protocadherin gamma subfamily B, 6 /// protocadherin gamma subfamily B, 7 /// protocadherin gamma subfamily C, 3 /// protocadherin gamma subfamily C, 4 /// protocadherin gamma subfamily C, 5" NM_0025885098PCDHGC3 PC43;PCDH-GAMMA-C3;PCDH2 "gb:NM_002588.1 /DEF=Homo sapiens protocadherin gamma subfamily C, 3 (PCDHGC3), mRNA. /FEA=mRNA /GEN=PCDHGC3 /PROD=protocadherin gamma subfamily C, 3 /DB_XREF=gi:4505632 /UG=Hs.173310 protocadherin gamma subfamily C, 3 /FL=gb:L11373.1 gb:NM_002588.1 gb:AF152337.1" 919.83 889.41 -1.03 -0.93 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 211066_x_at "protocadherin gamma subfamily A, 1 /// protocadherin gamma subfamily A, 10 /// protocadherin gamma subfamily A, 11 /// protocadherin gamma subfamily A, 12 /// protocadherin gamma subfamily A, 2 /// protocadherin gamma subfamily A, 3 /// protocadherin gamma subfamily A, 4 /// protocadherin gamma subfamily A, 5 /// protocadherin gamma subfamily A, 6 /// protocadherin gamma subfamily A, 7 /// protocadherin gamma subfamily A, 8 /// protocadherin gamma subfamily A, 9 /// protocadherin gamma subfamily B, 1 /// protocadherin gamma subfamily B, 2 /// protocadherin gamma subfamily B, 3 /// protocadherin gamma subfamily B, 4 /// protocadherin gamma subfamily B, 5 /// protocadherin gamma subfamily B, 6 /// protocadherin gamma subfamily B, 7 /// protocadherin gamma subfamily C, 3 /// protocadherin gamma subfamily C, 4 /// protocadherin gamma subfamily C, 5 /// protocadherin gamma subfamily A, 1 /// protocadherin gamma subfamily A, 10 /// protocadherin gamma subfamily A, 11 /// protocadherin gamma subfamily A, 12 /// protocadherin gamma subfamily A, 2 /// protocadherin gamma subfamily A, 3 /// protocadherin gamma subfamily A, 4 /// protocadherin gamma subfamily A, 5 /// protocadherin gamma subfamily A, 6 /// protocadherin gamma subfamily A, 7 /// protocadherin gamma subfamily A, 8 /// protocadherin gamma subfamily A, 9 /// protocadherin gamma subfamily B, 1 /// protocadherin gamma subfamily B, 2 /// protocadherin gamma subfamily B, 3 /// protocadherin gamma subfamily B, 4 /// protocadherin gamma subfamily B, 5 /// protocadherin gamma subfamily B, 6 /// protocadherin gamma subfamily B, 7 /// protocadherin gamma subfamily C, 3 /// protocadherin gamma subfamily C, 4 /// protocadherin gamma subfamily C, 5" BC0064395098PCDHGC3 PC43;PCDH-GAMMA-C3;PCDH2 "gb:BC006439.1 /DEF=Homo sapiens, Similar to protocadherin gamma subfamily A, 5, clone MGC:13163, mRNA, complete cds. /FEA=mRNA /PROD=Similar to protocadherin gamma subfamily A, 5 /DB_XREF=gi:13623638 /FL=gb:BC006439.1" 1449.4 1334.18 -1.09 -1 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.