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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 218978_s_at mitochondrial solute carrier protein NM_01858651312MSCP HT015;MSC;PRO1278;PRO1584 "gb:NM_018586.1 /DEF=Homo sapiens hypothetical protein PRO1584 (PRO1584), mRNA. /FEA=mRNA /GEN=PRO1584 /PROD=hypothetical protein PRO1584 /DB_XREF=gi:8924059 /UG=Hs.283716 hypothetical protein PRO1584 /FL=gb:AF116640.1 gb:NM_018586.1" 143.61 161.97 1.13 0.96 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 221920_s_at mitochondrial solute carrier protein BE67776151312MSCP HT015;MSC;PRO1278;PRO1584 Consensus includes gb:BE677761 /FEA=EST /DB_XREF=gi:10038376 /DB_XREF=est:7f59h05.x1 /CLONE=IMAGE:3299001 /UG=Hs.300496 mitochondrial solute carrier 258 266.97 1.03 0.89 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 218136_s_at mitochondrial solute carrier protein NM_01857951312MSCP HT015;MSC;PRO1278;PRO1584 "gb:NM_018579.1 /DEF=Homo sapiens mitochondrial solute carrier (LOC51312), mRNA. /FEA=mRNA /GEN=LOC51312 /PROD=hypothetical protein PRO1278 /DB_XREF=gi:8924027 /UG=Hs.300496 mitochondrial solute carrier /FL=gb:AF155660.1 gb:AF116630.1 gb:NM_018579.1" 324.12 310.71 -1.04 -0.97 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.