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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 218958_at hypothetical protein FLJ20850 NM_01796755049FLJ20850  "gb:NM_017967.1 /DEF=Homo sapiens hypothetical protein FLJ20850 (FLJ20850), mRNA. /FEA=mRNA /GEN=FLJ20850 /PROD=hypothetical protein FLJ20850 /DB_XREF=gi:8923696 /UG=Hs.30783 hypothetical protein FLJ20850 /FL=gb:NM_017967.1" 223.05 226.95 1.02 0.81 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 51200_at hypothetical protein FLJ20850 AI74408455049FLJ20850  "Cluster Incl. AI744084:wc36b05.x1 Homo sapiens cDNA, 3 end /clone=IMAGE-2317233 /clone_end=3' /gb=AI744084 /gi=5112372 /ug=Hs.30783 /len=524" 879.17 865.7 -1.02 -0.98 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.