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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 220134_x_at hypothetical protein FLJ10647 NM_01816655194FLJ10647  "gb:NM_018166.1 /DEF=Homo sapiens hypothetical protein FLJ10647 (FLJ10647), mRNA. /FEA=mRNA /GEN=FLJ10647 /PROD=hypothetical protein FLJ10647 /DB_XREF=gi:8922566 /UG=Hs.144391 hypothetical protein FLJ10647 /FL=gb:NM_018166.1" 222.65 255.91 1.15 1.03 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 221710_x_at hypothetical protein FLJ10647 /// hypothetical protein FLJ10647 BC00624155194FLJ10647  "gb:BC006241.1 /DEF=Homo sapiens, hypothetical protein FLJ10647, clone MGC:11318, mRNA, complete cds. /FEA=mRNA /PROD=hypothetical protein FLJ10647 /DB_XREF=gi:13623280 /FL=gb:BC006241.1" 210.08 227.6 1.08 0.86 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.