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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 209637_s_at regulator of G-protein signalling 12 AF0301116002RGS12  Consensus includes gb:AA887475 /FEA=EST /DB_XREF=gi:3003163 /DB_XREF=est:oj54a06.s1 /CLONE=IMAGE:1502098 /UG=Hs.78281 regulator of G-protein signalling 12 /FL=gb:AF030110.1 gb:AF030111.1 340.05 351.04 1.03 0.97 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 214362_at regulator of G-protein signalling 12 AW1706026002RGS12  Consensus includes gb:AW170602 /FEA=EST /DB_XREF=gi:6402127 /DB_XREF=est:xn64e03.x1 /CLONE=IMAGE:2698492 /UG=Hs.78281 regulator of G-protein signalling 12 117.68 156.1 1.33 0.95 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 209638_x_at "gb:AF030110.1 /DB_XREF=gi:2598195 /GEN=RGS /FEA=FLmRNA /CNT=90 /TID=Hs.78281.3 /TIER=ConsEnd /STK=0 /UG=Hs.78281 /LL=6002 /DEF=Homo sapiens regulator of G protein signaling RGS12 (RGS) mRNA, complete cds. /PROD=regulator of G protein signaling RGS12 /FL=gb:AF030111.1 gb:AF030110.1" AF0301106002RGS12  "gb:AF030110.1 /DB_XREF=gi:2598195 /GEN=RGS /FEA=FLmRNA /CNT=90 /TID=Hs.78281.3 /TIER=ConsEnd /STK=0 /UG=Hs.78281 /LL=6002 /DEF=Homo sapiens regulator of G protein signaling RGS12 (RGS) mRNA, complete cds. /PROD=regulator of G protein signaling RGS12 /FL=gb:AF030111.1 gb:AF030110.1" 132.43 122.17 -1.08 -0.72 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 209639_s_at regulator of G-protein signalling 12 AF0301116002RGS12  "gb:AF030111.1 /DB_XREF=gi:2599396 /GEN=RGS /FEA=FLmRNA /CNT=90 /TID=Hs.78281.3 /TIER=FL /STK=7 /UG=Hs.78281 /LL=6002 /DEF=Homo sapiens regulator of G protein signaling RGS12 (RGS) mRNA, complete cds. /PROD=regulator of G protein signaling /FL=gb:AF030111.1 gb:AF030110.1" 141.86 127.17 -1.12 -0.88 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
5 205823_at regulator of G-protein signalling 12 AI8241136002RGS12  gb:AI824113 /DB_XREF=gi:5444784 /DB_XREF=wj46d03.x1 /CLONE=IMAGE:2405861 /FEA=FLmRNA /CNT=29 /TID=Hs.78281.0 /TIER=Stack /STK=14 /UG=Hs.78281 /LL=6002 /UG_GENE=RGS12 /UG_TITLE=regulator of G-protein signalling 12 /FL=gb:AF035152.1 gb:NM_002926.1 183.97 178.52 -1.03 -0.89 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
6 214361_s_at regulator of G-protein signalling 12 AW1706026002RGS12  gb:AW170602 /DB_XREF=gi:6402127 /DB_XREF=xn64e03.x1 /CLONE=IMAGE:2698492 /FEA=EST /CNT=11 /TID=Hs.78281.4 /TIER=Stack /STK=9 /UG=Hs.78281 /LL=6002 /UG_GENE=RGS12 /UG_TITLE=regulator of G-protein signalling 12 289.1 272.25 -1.06 -0.93 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.