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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 214167_s_at "ribosomal protein, large, P0" AA5551136175RPLP0 L10E;P0;PRLP0;RPP0 "gb:AA555113 /DB_XREF=gi:2325652 /DB_XREF=nj40g05.s1 /CLONE=IMAGE:995000 /FEA=EST /CNT=12 /TID=Hs.73742.4 /TIER=Stack /STK=12 /UG=Hs.73742 /LL=6175 /UG_GENE=RPLP0 /UG_TITLE=ribosomal protein, large, P0" 5304.55 5529.74 1.04 0.99 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 201033_x_at "ribosomal protein, large, P0" NM_0010026175RPLP0 L10E;P0;PRLP0;RPP0 "gb:NM_001002.1 /DEF=Homo sapiens ribosomal protein, large, P0 (RPLP0), mRNA. /FEA=mRNA /GEN=RPLP0 /PROD=ribosomal protein, large, P0 /DB_XREF=gi:4506666 /UG=Hs.73742 ribosomal protein, large, P0 /FL=gb:BC000345.1 gb:BC000752.1 gb:BC001127.1 gb:AF274958.1 gb:BC001834.1 gb:M17885.1 gb:NM_001002.1" 6639.26 6882.2 1.04 0.98 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 208856_x_at "ribosomal protein, large, P0" BC0036556175RPLP0 L10E;P0;PRLP0;RPP0 "gb:BC003655.1 /DB_XREF=gi:13177771 /FEA=FLmRNA /CNT=221 /TID=Hs.73742.1 /TIER=FL /STK=5 /UG=Hs.73742 /LL=6175 /UG_GENE=RPLP0 /DEF=Homo sapiens, ribosomal protein, large, P0, clone MGC:4770, mRNA, complete cds. /PROD=ribosomal protein, large, P0 /FL=gb:BC003655.1 gb:BC000087.1" 6731 6895.99 1.02 0.98 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 211720_x_at "ribosomal protein, large, P0 /// ribosomal protein, large, P0" BC0058636175RPLP0 L10E;P0;PRLP0;RPP0 "gb:BC005863.1 /DB_XREF=gi:13543411 /FEA=FLmRNA /CNT=1 /TID=HsAffx.900725.928 /TIER=FL /STK=0 /DEF=Homo sapiens, ribosomal protein, large, P0, clone MGC:3679, mRNA, complete cds. /PROD=ribosomal protein, large, P0 /FL=gb:BC005863.1" 6772.2 6816.63 1.01 0.97 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
5 211972_x_at "ribosomal protein, large, P0" AI9538226175RPLP0 L10E;P0;PRLP0;RPP0 "gb:AI953822 /DB_XREF=gi:5746132 /DB_XREF=wx69h05.x1 /CLONE=IMAGE:2548953 /FEA=EST /CNT=494 /TID=Hs.73742.3 /TIER=Stack /STK=53 /UG=Hs.73742 /LL=6175 /UG_GENE=RPLP0 /UG_TITLE=ribosomal protein, large, P0" 6192.3 6256.13 1.01 0.96 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
6 211526_s_at chromosome 20 open reading frame 41 BC0006736175RPLP0 L10E;P0;PRLP0;RPP0 "gb:BC000673.1 /DEF=Homo sapiens, Similar to helicase-like protein NHL, clone MGC:665, mRNA, complete cds. /FEA=mRNA /PROD=Similar to helicase-like protein NHL /DB_XREF=gi:12653774 /UG=Hs.73742 ribosomal protein, large, P0 /FL=gb:BC000673.1" 59.23 42.28 -1.4 -0.83 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.