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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 207852_at chemokine (C-X-C motif) ligand 5 NM_0029946374CXCL5 ENA-78;ENA78;SCYB5 "gb:NM_002994.1 /DB_XREF=gi:4506848 /GEN=SCYB5 /FEA=FLmRNA /CNT=3 /TID=Hs.89714.0 /TIER=FL /STK=0 /UG=Hs.89714 /LL=6374 /DEF=Homo sapiens small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78) (SCYB5), mRNA. /PROD=small inducible cytokine subfamily B(Cys-X-Cys), member 5 (epithelial-derivedneutrophil-activating peptide 78) /FL=gb:NM_002994.1" 232.16 248.18 1.07 1 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 214974_x_at chemokine (C-X-C motif) ligand 5 AK0265466374CXCL5 ENA-78;ENA78;SCYB5 "Consensus includes gb:AK026546.1 /DEF=Homo sapiens cDNA: FLJ22893 fis, clone KAT04792. /FEA=mRNA /DB_XREF=gi:10439427 /UG=Hs.287716 Homo sapiens cDNA: FLJ22893 fis, clone KAT04792" 577.62 606.55 1.05 0.92 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 215101_s_at chemokine (C-X-C motif) ligand 5 BG1667056374CXCL5 ENA-78;ENA78;SCYB5 "gb:BG166705 /DB_XREF=gi:12673408 /DB_XREF=602339217F1 /CLONE=IMAGE:4447013 /FEA=DNA /CNT=9 /TID=Hs.89714.1 /TIER=ConsEnd /STK=0 /UG=Hs.89714 /LL=6374 /UG_GENE=SCYB5 /UG_TITLE=small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78)" 164.1 166.69 1.02 0.85 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.