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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 221284_s_at v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) NM_0054176714SRC ASV;SRC1;c-SRC;p60-Src "gb:NM_005417.1 /DEF=Homo sapiens v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (SRC), mRNA. /FEA=CDS /GEN=SRC /PROD=v-src avian sarcoma (Schmidt-Ruppin A-2) viraloncogene homolog /DB_XREF=gi:4885608 /UG=Hs.198298 v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog /FL=gb:NM_005417.1" 130.02 134.99 1.04 0.87 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 221281_at "Consensus includes gb:NM_005417.1 /DEF=Homo sapiens v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (SRC), mRNA. /FEA=CDS /GEN=SRC /PROD=v-src avian sarcoma (Schmidt-Ruppin A-2) viraloncogene homolog /DB_XREF=gi:4885608 /UG=Hs.198298 v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog /FL=gb:NM_005417.1" NM_0054176714SRC ASV;SRC1;c-SRC;p60-Src "Consensus includes gb:NM_005417.1 /DEF=Homo sapiens v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (SRC), mRNA. /FEA=CDS /GEN=SRC /PROD=v-src avian sarcoma (Schmidt-Ruppin A-2) viraloncogene homolog /DB_XREF=gi:4885608 /UG=Hs.198298 v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog /FL=gb:NM_005417.1" 83.77 87.58 1.05 0.56 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 213324_at v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) AK0242816714SRC ASV;SRC1;c-SRC;p60-Src "gb:AK024281.1 /DB_XREF=gi:10436622 /FEA=mRNA /CNT=54 /TID=Hs.198298.0 /TIER=Stack /STK=20 /UG=Hs.198298 /LL=6714 /UG_GENE=SRC /UG_TITLE=v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog /DEF=Homo sapiens cDNA FLJ14219 fis, clone NT2RP3003800, highly similar to Rattus norvegicus tyrosine protein kinase pp60-c-src mRNA." 365.61 361.21 -1.01 -0.93 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.