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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 213535_s_at "ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)" AA9106147329UBE2I C358B7.1;P18;UBC9 gb:AA910614 /DB_XREF=gi:3049904 /DB_XREF=ok61b04.s1 /CLONE=IMAGE:1518415 /FEA=EST /CNT=42 /TID=Hs.84285.2 /TIER=ConsEnd /STK=0 /UG=Hs.84285 /LL=7329 /UG_GENE=UBE2I /UG_TITLE=ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9) 1069.54 1167.37 1.09 1.02 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 211008_s_at "ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)" BC0007447329UBE2I C358B7.1;P18;UBC9 "gb:BC000744.1 /DB_XREF=gi:12653902 /FEA=FLmRNA /CNT=3 /TID=Hs.84285.1 /TIER=FL /STK=0 /UG=Hs.84285 /LL=7329 /UG_GENE=UBE2I /DEF=Homo sapiens, Similar to ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9), clone MGC:2377, mRNA, complete cds. /PROD=Similar to ubiquitin-conjugating enzyme E2I(homologous to yeast UBC9) /FL=gb:BC000744.1 gb:BC004437.1" 148.37 154.51 1.04 0.82 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 213536_s_at gb:AA910614 /DB_XREF=gi:3049904 /DB_XREF=ok61b04.s1 /CLONE=IMAGE:1518415 /FEA=EST /CNT=42 /TID=Hs.84285.2 /TIER=Stack /STK=12 /UG=Hs.84285 /LL=7329 /UG_GENE=UBE2I /UG_TITLE=ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9) AA9106147329UBE2I C358B7.1;P18;UBC9 gb:AA910614 /DB_XREF=gi:3049904 /DB_XREF=ok61b04.s1 /CLONE=IMAGE:1518415 /FEA=EST /CNT=42 /TID=Hs.84285.2 /TIER=Stack /STK=12 /UG=Hs.84285 /LL=7329 /UG_GENE=UBE2I /UG_TITLE=ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9) 60.64 70.93 1.17 0.75 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 208760_at "ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)" AL0317147329UBE2I C358B7.1;P18;UBC9 "gb:AL031714 /DB_XREF=gi:4775608 /FEA=FLmRNA /CNT=328 /TID=Hs.84285.0 /TIER=Stack /STK=30 /UG=Hs.84285 /LL=7329 /UG_GENE=UBE2I /UG_TITLE=ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9) /DEF=Human DNA sequence from clone LA16-358B7 on chromosome 16 Contains the UBE21 gene for ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9), and an RPS20 (40S Ribosomal protein S20) pseudogene. Contains ESTs, STSs. GSSs and a putative CpG is... /FL=gb:U31933.1 gb:U66867.1 gb:U38785.1 gb:U66818.1 gb:NM_003345.1 gb:BC000427.1 gb:BC004429.1 gb:U31882.1 gb:U45328.1 gb:U29092.1" 435.15 314.39 -1.38 -1.2 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.