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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 205277_at "PR domain containing 2, with ZNF domain" NM_0122317799PRDM2 HUMHOXY1;MTB-ZF;RIZ;RIZ1;RIZ2 "gb:NM_012231.1 /DB_XREF=gi:10092605 /GEN=PRDM2 /FEA=FLmRNA /CNT=37 /TID=Hs.289024.0 /TIER=FL+Stack /STK=9 /UG=Hs.289024 /LL=7799 /DEF=Homo sapiens PR domain containing 2, with ZNF domain (PRDM2), mRNA. /PROD=PR domain containing 2, with ZNF domain /FL=gb:NM_012231.1 gb:U17838.1" 315.04 374.3 1.19 0.99 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 203056_s_at "PR domain containing 2, with ZNF domain" NM_0158667799PRDM2 HUMHOXY1;MTB-ZF;RIZ;RIZ1;RIZ2 Consensus includes gb:AI681013 /FEA=EST /DB_XREF=gi:4891195 /DB_XREF=est:tx43d04.x1 /CLONE=IMAGE:2272327 /UG=Hs.26719 zinc-finger DNA-binding protein /FL=gb:D45132.1 gb:NM_015866.1 276.87 272.95 -1.01 -0.83 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 216445_at "Consensus includes gb:U23736.1 /DEF=Human GATA-3 binding protein G3B mRNA, partial cds. /FEA=mRNA /PROD=GATA-3 binding protein G3B /DB_XREF=gi:915214 /UG=Hs.289024 PR domain containing 2, with ZNF domain" U237367799PRDM2 HUMHOXY1;MTB-ZF;RIZ;RIZ1;RIZ2 "Consensus includes gb:U23736.1 /DEF=Human GATA-3 binding protein G3B mRNA, partial cds. /FEA=mRNA /PROD=GATA-3 binding protein G3B /DB_XREF=gi:915214 /UG=Hs.289024 PR domain containing 2, with ZNF domain" 76.3 58.59 -1.3 -0.9 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 203057_s_at "PR domain containing 2, with ZNF domain" AV7247837799PRDM2 HUMHOXY1;MTB-ZF;RIZ;RIZ1;RIZ2 gb:AV724783 /DB_XREF=gi:10829513 /DB_XREF=AV724783 /CLONE=HTBCEG11 /FEA=FLmRNA /CNT=109 /TID=Hs.26719.0 /TIER=Stack /STK=17 /UG=Hs.26719 /LL=51357 /UG_GENE=HUMHOXY1 /UG_TITLE=zinc-finger DNA-binding protein /FL=gb:NM_015866.1 gb:D45132.1 304.99 288.14 -1.06 -0.92 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
5 216433_s_at "PR domain containing 2, with ZNF domain" U237367799PRDM2 HUMHOXY1;MTB-ZF;RIZ;RIZ1;RIZ2 "Consensus includes gb:U23736.1 /DEF=Human GATA-3 binding protein G3B mRNA, partial cds. /FEA=mRNA /PROD=GATA-3 binding protein G3B /DB_XREF=gi:915214 /UG=Hs.289024 PR domain containing 2, with ZNF domain" 446.05 189.12 -2.36 -1.55 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.