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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 205634_x_at hypothetical protein LOC254359 NM_02429879143LENG4 BB1 "gb:NM_024298.1 /DB_XREF=gi:13236521 /GEN=LENG4 /FEA=FLmRNA /CNT=35 /TID=Hs.78768.0 /TIER=FL /STK=0 /UG=Hs.78768 /LL=79143 /DEF=Homo sapiens malignant cell expression-enhanced genetumor progression-enhanced gene (LENG4), mRNA. /PROD=malignant cell expression-enhanced genetumorprogression-enhanced gene /FL=gb:BC002598.1 gb:NM_024298.1" 240.8 241.21 1 0.87 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 211037_s_at leukocyte receptor cluster (LRC) member 4 /// leukocyte receptor cluster (LRC) member 4 BC00630979143LENG4 BB1 "gb:BC006309.1 /DEF=Homo sapiens, Similar to RIKEN cDNA 5730589L02 gene, clone MGC:13124, mRNA, complete cds. /FEA=mRNA /PROD=Similar to RIKEN cDNA 5730589L02 gene /DB_XREF=gi:13623420 /FL=gb:BC006309.1" 158.04 149.81 -1.05 -0.86 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 209179_s_at leukocyte receptor cluster (LRC) member 4 BC00316479143LENG4 BB1 "gb:BC003164.1 /DB_XREF=gi:13111982 /FEA=FLmRNA /CNT=157 /TID=Hs.78768.1 /TIER=FL /STK=0 /UG=Hs.78768 /LL=79143 /UG_GENE=LENG4 /DEF=Homo sapiens, clone MGC:4221, mRNA, complete cds. /PROD=Unknown (protein for MGC:4221) /FL=gb:BC002512.1 gb:BC003164.1" 600.22 543.52 -1.1 -1.01 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.