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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 211581_x_at leukocyte specific transcript 1 AF0004267940LST1 B144;D6S49E;LST-1 "gb:AF000426.1 /DEF=Homo sapiens LST1 mRNA, cLST1E splice variant, complete cds. /FEA=mRNA /GEN=LST1 /DB_XREF=gi:2145067 /UG=Hs.88411 lymphocyte antigen 117 /FL=gb:AF000426.1" 193.23 253.01 1.31 1.1 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 211582_x_at leukocyte specific transcript 1 AF0004247940LST1 B144;D6S49E;LST-1 "gb:AF000424.1 /DB_XREF=gi:2145063 /GEN=LST1 /FEA=FLmRNA /CNT=2 /TID=Hs.88411.2 /TIER=FL /STK=0 /UG=Hs.88411 /LL=7940 /UG_TITLE=lymphocyte antigen 117 /DEF=Homo sapiens LST1 mRNA, cLST1C splice variant, complete cds. /FL=gb:AF000424.1" 454.84 520.9 1.15 0.91 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 210629_x_at leukocyte specific transcript 1 AF0004257940LST1 B144;D6S49E;LST-1 "gb:AF000425.1 /DEF=Homo sapiens LST1 mRNA, cLST1A splice variant, complete cds. /FEA=mRNA /GEN=LST1 /DB_XREF=gi:2145065 /UG=Hs.88411 lymphocyte antigen 117 /FL=gb:AF000425.1" 264.49 267.51 1.01 0.81 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 214181_x_at leukocyte specific transcript 1 AI7356927940LST1 B144;D6S49E;LST-1 Consensus includes gb:AI735692 /FEA=EST /DB_XREF=gi:5057216 /DB_XREF=est:at20b12.x1 /CLONE=IMAGE:2355647 /UG=Hs.88411 lymphocyte antigen 117 114.31 93.8 -1.22 -0.74 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
5 215633_x_at leukocyte specific transcript 1 AV7137207940LST1 B144;D6S49E;LST-1 Consensus includes gb:AV713720 /FEA=EST /DB_XREF=gi:10795237 /DB_XREF=est:AV713720 /CLONE=DCBBJA12 /UG=Hs.306434 Homo sapiens mRNA for LST-1N protein 232.97 229.64 -1.01 -0.84 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
6 214574_x_at leukocyte specific transcript 1 NM_0071617940LST1 B144;D6S49E;LST-1 "Consensus includes gb:NM_007161.1 /DEF=Homo sapiens DNA segment on chromosome 6 (unique) 49 expressed sequence, NK cell triggering receptor, p30 (D6S49E), mRNA. /FEA=CDS /GEN=D6S49E /PROD=leukocyte-specific transcript 1 /DB_XREF=gi:6005740 /UG=Hs.88411 lymphocyte antigen 117 /FL=gb:NM_007161.1" 392.81 364.4 -1.08 -0.91 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.