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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 200755_s_at calumenin BF939365813CALU  gb:BF939365 /DB_XREF=gi:12356685 /DB_XREF=nad87h04.x1 /CLONE=IMAGE:3410551 /FEA=FLmRNA /CNT=452 /TID=Hs.7753.0 /TIER=Stack /STK=19 /UG=Hs.7753 /LL=813 /UG_GENE=CALU /UG_TITLE=calumenin /FL=gb:AF013759.1 gb:U67280.1 gb:NM_001219.2 712.75 769.17 1.08 0.98 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 200756_x_at calumenin U67280813CALU  "gb:U67280.1 /DB_XREF=gi:2809323 /FEA=FLmRNA /CNT=452 /TID=Hs.7753.0 /TIER=FL /STK=0 /UG=Hs.7753 /LL=813 /UG_GENE=CALU /DEF=Homo sapiens calumenin mRNA, complete cds. /PROD=calumenin /FL=gb:AF013759.1 gb:U67280.1 gb:NM_001219.2" 480.02 487.99 1.02 0.93 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 214845_s_at calumenin AF257659813CALU  "gb:AF257659.1 /DB_XREF=gi:8515717 /FEA=mRNA /CNT=11 /TID=Hs.302073.0 /TIER=ConsEnd /STK=0 /UG=Hs.302073 /DEF=Homo sapiens crocalbin-like protein mRNA, partial cds. /PROD=crocalbin-like protein" 995.05 979.96 -1.02 -0.95 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 200757_s_at calumenin NM_001219813CALU  "gb:NM_001219.2 /DB_XREF=gi:6005991 /GEN=CALU /FEA=FLmRNA /CNT=452 /TID=Hs.7753.0 /TIER=FL+Stack /STK=69 /UG=Hs.7753 /LL=813 /DEF=Homo sapiens calumenin (CALU), mRNA. /PROD=calumenin precursor /FL=gb:AF013759.1 gb:U67280.1 gb:NM_001219.2" 1898.67 1720.71 -1.1 -1.05 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.