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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 204442_x_at latent transforming growth factor beta binding protein 4 NM_0035738425LTBP4 LTBP-4 "gb:NM_003573.1 /DEF=Homo sapiens latent transforming growth factor beta binding protein 4 (LTBP4), mRNA. /FEA=mRNA /GEN=LTBP4 /PROD=latent transforming growth factor beta bindingprotein 4 /DB_XREF=gi:4505036 /UG=Hs.85087 latent transforming growth factor beta binding protein 4 /FL=gb:NM_003573.1" 130.02 141.24 1.09 0.82 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 210628_x_at latent transforming growth factor beta binding protein 4 AF0513448425LTBP4 LTBP-4 "gb:AF051344.1 /DB_XREF=gi:3327807 /FEA=FLmRNA /CNT=4 /TID=Hs.85087.1 /TIER=FL /STK=0 /UG=Hs.85087 /LL=8425 /UG_GENE=LTBP4 /DEF=Homo sapiens latent transforming growth factor-beta binding protein 4S mRNA, complete cds. /PROD=latent transforming growth factor-beta bindingprotein 4S /FL=gb:AF051344.1" 334.8 351.92 1.05 0.78 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 213176_s_at latent transforming growth factor beta binding protein 4 AI9108698425LTBP4 LTBP-4 Consensus includes gb:AI910869 /FEA=EST /DB_XREF=gi:5630605 /DB_XREF=est:wd20f04.x1 /CLONE=IMAGE:2328703 /UG=Hs.85087 latent transforming growth factor beta binding protein 4 185.88 158.03 -1.18 -0.89 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.