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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 210257_x_at cullin 4B AF2129958450CUL4B KIAA0695 "gb:AF212995.1 /DB_XREF=gi:13259126 /GEN=CUL4B /FEA=FLmRNA /CNT=10 /TID=Hs.155976.1 /TIER=FL /STK=0 /UG=Hs.155976 /LL=8450 /DEF=Homo sapiens cullin CUL4B (CUL4B) mRNA, complete cds. /PROD=cullin CUL4B /FL=gb:AF212995.1" 358.21 387.04 1.08 0.99 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 202213_s_at cullin 4B AI6508198450CUL4B KIAA0695 gb:AI650819 /DB_XREF=gi:4734798 /DB_XREF=wa95a10.x1 /CLONE=IMAGE:2303898 /FEA=FLmRNA /CNT=168 /TID=Hs.155976.0 /TIER=Stack /STK=10 /UG=Hs.155976 /LL=8450 /UG_GENE=CUL4B /UG_TITLE=cullin 4B /FL=gb:AB014595.1 gb:NM_003588.1 593.91 626.15 1.05 0.98 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 215997_s_at cullin 4B AV6947328450CUL4B KIAA0695 Consensus includes gb:AV694732 /FEA=EST /DB_XREF=gi:10296595 /DB_XREF=est:AV694732 /CLONE=GKCGZH06 /UG=Hs.155976 cullin 4B 322.67 339.4 1.05 0.95 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 202214_s_at cullin 4B NM_0035888450CUL4B KIAA0695 "gb:NM_003588.1 /DB_XREF=gi:13270466 /GEN=CUL4B /FEA=FLmRNA /CNT=168 /TID=Hs.155976.0 /TIER=FL+Stack /STK=48 /UG=Hs.155976 /LL=8450 /DEF=Homo sapiens cullin 4B (CUL4B), mRNA. /PROD=cullin 4B /FL=gb:AB014595.1 gb:NM_003588.1" 1749.37 1450.31 -1.21 -1.11 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.