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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 210648_x_at sorting nexin 3 AB0473608724SNX3 MGC17570;SDP3;SNX3A "gb:AB047360.1 /DB_XREF=gi:12957159 /GEN=SNX 3A /FEA=FLmRNA /CNT=3 /TID=Hs.12102.1 /TIER=FL /STK=0 /UG=Hs.12102 /LL=8724 /DEF=Homo sapiens SNX 3A mRNA for sorting nexin 3A, complete cds. /PROD=sorting nexin 3A /FL=gb:AB047360.1" 2738.03 2886.08 1.05 1.01 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 213545_x_at sorting nexin 3 BE9626158724SNX3 MGC17570;SDP3;SNX3A gb:BE962615 /DB_XREF=gi:11765691 /DB_XREF=601655968R1 /CLONE=IMAGE:3855785 /FEA=EST /CNT=30 /TID=Hs.12102.2 /TIER=Stack /STK=19 /UG=Hs.12102 /LL=8724 /UG_GENE=SNX3 /UG_TITLE=sorting nexin 3 1513.52 1636.86 1.08 1.01 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 200067_x_at "Consensus includes gb:AL078596 /DEF=Human DNA sequence from clone RP3-429G5 on chromosome 6q21-22.1. Contains the NR2E1 gene for nuclear receptor 2E1 (tailless, TLL, TLX, XTLL), the 3 end of the SNX3 gene for sorting nexin 3, ESTs, STSs, GSSs and four predicted CpG islands /FEA=mRNA_2 /DB_XREF=gi:6010168 /UG=Hs.12102 sorting nexin 3 /FL=gb:AF062483.1 gb:AF034546.1 gb:NM_003795.1" AL0785968724SNX3 MGC17570;SDP3;SNX3A "Consensus includes gb:AL078596 /DEF=Human DNA sequence from clone RP3-429G5 on chromosome 6q21-22.1. Contains the NR2E1 gene for nuclear receptor 2E1 (tailless, TLL, TLX, XTLL), the 3 end of the SNX3 gene for sorting nexin 3, ESTs, STSs, GSSs and four predicted CpG islands /FEA=mRNA_2 /DB_XREF=gi:6010168 /UG=Hs.12102 sorting nexin 3 /FL=gb:AF062483.1 gb:AF034546.1 gb:NM_003795.1" 1711.56 1810.93 1.06 1 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 208781_x_at sorting nexin 3 AL0785968724SNX3 MGC17570;SDP3;SNX3A "gb:AF062483.1 /DEF=Homo sapiens SDP3 mRNA, complete cds. /FEA=mRNA /PROD=SDP3 /DB_XREF=gi:3126978 /UG=Hs.12102 sorting nexin 3 /FL=gb:AF062483.1 gb:AF034546.1 gb:NM_003795.1" 1693.17 1686.3 -1 -0.94 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.