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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 204208_at RNA guanylyltransferase and 5'-phosphatase NM_0038008732RNGTT CAP1A;HCE;HCE1;hCAP "gb:NM_003800.1 /DB_XREF=gi:4506562 /GEN=RNGTT /FEA=FLmRNA /CNT=91 /TID=Hs.27345.0 /TIER=FL+Stack /STK=39 /UG=Hs.27345 /LL=8732 /DEF=Homo sapiens RNA guanylyltransferase and 5-phosphatase (RNGTT), mRNA. /PROD=RNA guanylyltransferase and 5-phosphatase /FL=gb:AB012142.1 gb:AB009022.1 gb:AF025654.1 gb:NM_003800.1" 109.79 125.29 1.14 1.02 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 211387_x_at RNA guanylyltransferase and 5'-phosphatase AB0121438732RNGTT CAP1A;HCE;HCE1;hCAP "gb:AB012143.1 /DB_XREF=gi:2979497 /GEN=hCAP1b /FEA=FLmRNA /CNT=1 /TID=Hs.27345.1 /TIER=FL /STK=0 /UG=Hs.27345 /LL=8732 /DEF=Homo sapiens hCAP1b mRNA for mRNA capping enzyme, complete cds. /PROD=mRNA capping enzyme /FL=gb:AB012143.1" 65.38 63.92 -1.02 -0.72 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 204207_s_at RNA guanylyltransferase and 5'-phosphatase AB0121428732RNGTT CAP1A;HCE;HCE1;hCAP "gb:AB012142.1 /DB_XREF=gi:2979495 /GEN=hCAP1a /FEA=FLmRNA /CNT=91 /TID=Hs.27345.0 /TIER=FL /STK=0 /UG=Hs.27345 /LL=8732 /DEF=Homo sapiens hCAP1a mRNA for mRNA capping enzyme, complete cds. /PROD=mRNA capping enzyme /FL=gb:AB012142.1 gb:AB009022.1 gb:AF025654.1 gb:NM_003800.1" 229.79 218.21 -1.05 -0.91 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 211849_s_at RNA guanylyltransferase and 5'-phosphatase AB0090238732RNGTT CAP1A;HCE;HCE1;hCAP "gb:AB009023.1 /DB_XREF=gi:3097309 /GEN=HCE1A /FEA=FLmRNA /CNT=2 /TID=Hs.27345.2 /TIER=FL /STK=0 /UG=Hs.27345 /LL=8732 /DEF=Homo sapiens HCE1A mRNA for capping enzyme 1A, complete cds. /PROD=capping enzyme 1A /FL=gb:AB009024.1 gb:AB009023.1" 117.99 107.92 -1.09 -0.93 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.