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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 202769_at cyclin G2 AW134535901CCNG2  gb:AW134535 /DB_XREF=gi:6138088 /DB_XREF=UI-H-BI1-abv-g-06-0-UI.s1 /CLONE=IMAGE:2713163 /FEA=FLmRNA /CNT=129 /TID=Hs.79069.0 /TIER=Stack /STK=45 /UG=Hs.79069 /LL=901 /UG_GENE=CCNG2 /UG_TITLE=cyclin G2 /FL=gb:NM_004354.1 gb:U47414.1 1080.17 1768.93 1.64 1.55 "all, CREBtarget" HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 211559_s_at cyclin G2 L49506901CCNG2  "gb:L49506.1 /DEF=Homo sapiens cyclin G2 mRNA, complete cds. /FEA=mRNA /PROD=cyclin G2 /DB_XREF=gi:1236234 /UG=Hs.79069 cyclin G2 /FL=gb:L49506.1" 570.88 865.43 1.52 1.38 "all, CREBtarget" HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 202770_s_at cyclin G2 NM_004354901CCNG2  "gb:NM_004354.1 /DB_XREF=gi:4757935 /GEN=CCNG2 /FEA=FLmRNA /CNT=129 /TID=Hs.79069.0 /TIER=FL /STK=0 /UG=Hs.79069 /LL=901 /DEF=Homo sapiens cyclin G2 (CCNG2), mRNA. /PROD=cyclin G2 /FL=gb:NM_004354.1 gb:U47414.1" 1053.39 1458.23 1.38 1.26 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.