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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 212470_at sperm associated antigen 9 AB0110889043SPAG9 FLJ13450;FLJ14006;FLJ34602;HSS;JLP;KIAA0516;MGC14967;PHET "gb:AB011088.1 /DB_XREF=gi:3043555 /GEN=KIAA0516 /FEA=mRNA /CNT=150 /TID=Hs.129872.1 /TIER=Stack /STK=50 /UG=Hs.129872 /LL=9043 /DEF=Homo sapiens mRNA for KIAA0516 protein, partial cds. /PROD=KIAA0516 protein" 1104.61 1155.07 1.05 0.96 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 206748_s_at sperm associated antigen 9 NM_0039719043SPAG9 FLJ13450;FLJ14006;FLJ34602;HSS;JLP;KIAA0516;MGC14967;PHET "gb:NM_003971.1 /DEF=Homo sapiens sperm associated antigen 9 (SPAG9), mRNA. /FEA=mRNA /GEN=SPAG9 /PROD=sperm associated antigen 9 /DB_XREF=gi:4504524 /UG=Hs.129872 sperm associated antigen 9 /FL=gb:NM_003971.1" 349.1 359.32 1.03 0.94 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 212468_at sperm associated antigen 9 AK0235129043SPAG9 FLJ13450;FLJ14006;FLJ34602;HSS;JLP;KIAA0516;MGC14967;PHET "gb:AK023512.1 /DB_XREF=gi:10435467 /FEA=mRNA /CNT=150 /TID=Hs.129872.1 /TIER=Stack /STK=16 /UG=Hs.129872 /LL=9043 /UG_GENE=SPAG9 /UG_TITLE=sperm associated antigen 9 /DEF=Homo sapiens cDNA FLJ13450 fis, clone PLACE1003027, highly similar to Homo sapiens mRNA for KIAA0516 protein." 281.86 198.55 -1.42 -1.24 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.