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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 202610_s_at "cofactor required for Sp1 transcriptional activation, subunit 2, 150kDa" AF1358029282CRSP2 CRSP150;CSRP;CXORF4;DRIP150;EXLM1;RGR1;TRAP170 "gb:AF135802.1 /DB_XREF=gi:4580325 /FEA=FLmRNA /CNT=258 /TID=Hs.21586.0 /TIER=FL+Stack /STK=9 /UG=Hs.21586 /LL=9282 /UG_GENE=CRSP2 /DEF=Homo sapiens thyroid hormone receptor-associated protein complex component TRAP170 mRNA, complete cds. /PROD=thyroid hormone receptor-associated proteincomplex component TRAP170 /FL=gb:AF304448.1 gb:AB006651.1 gb:AF135802.1 gb:NM_004229.1 gb:AF104256.1" 590.45 608.12 1.03 0.96 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 202611_s_at "cofactor required for Sp1 transcriptional activation, subunit 2, 150kDa" NM_0042299282CRSP2 CRSP150;CSRP;CXORF4;DRIP150;EXLM1;RGR1;TRAP170 "Consensus includes gb:AI971089 /FEA=EST /DB_XREF=gi:5767915 /DB_XREF=est:wr23g11.x1 /CLONE=IMAGE:2488580 /UG=Hs.21586 cofactor required for Sp1 transcriptional activation, subunit 2 (150kD) /FL=gb:AF304448.1 gb:AB006651.1 gb:AF104256.1 gb:AF135802.1 gb:NM_004229.1" 161.43 187.58 1.16 0.96 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 215167_at "cofactor required for Sp1 transcriptional activation, subunit 2, 150kDa" BE5670329282CRSP2 CRSP150;CSRP;CXORF4;DRIP150;EXLM1;RGR1;TRAP170 "Consensus includes gb:BE567032 /FEA=EST /DB_XREF=gi:9810752 /DB_XREF=est:601341180F1 /CLONE=IMAGE:3683563 /UG=Hs.21586 cofactor required for Sp1 transcriptional activation, subunit 2 (150kD)" 215.22 221.91 1.03 0.86 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 202612_s_at "cofactor required for Sp1 transcriptional activation, subunit 2, 150kDa" NM_0042299282CRSP2 CRSP150;CSRP;CXORF4;DRIP150;EXLM1;RGR1;TRAP170 "gb:NM_004229.1 /DB_XREF=gi:4758101 /GEN=CRSP2 /FEA=FLmRNA /CNT=258 /TID=Hs.21586.0 /TIER=FL /STK=0 /UG=Hs.21586 /LL=9282 /DEF=Homo sapiens cofactor required for Sp1 transcriptional activation, subunit 2 (150kD) (CRSP2), mRNA. /PROD=cofactor required for Sp1 transcriptionalactivation, subunit 2 (150kD) /FL=gb:AF304448.1 gb:AB006651.1 gb:AF135802.1 gb:NM_004229.1 gb:AF104256.1" 44.74 39.21 -1.14 -0.75 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
5 217120_s_at "Consensus includes gb:AK023368.1 /DEF=Homo sapiens cDNA FLJ13306 fis, clone OVARC1001417, highly similar to Homo sapiens thyroid hormone receptor-associated protein complex component TRAP170 mRNA. /FEA=mRNA /DB_XREF=gi:10435273 /UG=Hs.21586 cofactor required for Sp1 transcriptional activation, subunit 2 (150kD)" AK0233689282CRSP2 CRSP150;CSRP;CXORF4;DRIP150;EXLM1;RGR1;TRAP170 "Consensus includes gb:AK023368.1 /DEF=Homo sapiens cDNA FLJ13306 fis, clone OVARC1001417, highly similar to Homo sapiens thyroid hormone receptor-associated protein complex component TRAP170 mRNA. /FEA=mRNA /DB_XREF=gi:10435273 /UG=Hs.21586 cofactor required for Sp1 transcriptional activation, subunit 2 (150kD)" 157.7 128.89 -1.22 -0.98 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.