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Tip: To quickly search the same gene in another data set, click the links from the last column. The locuslink number will be used as query.
|No||probe set||Gene Name||Accession||LocusLink||Symbol||Alias Symbols||Description||CTRL||FSK||(FSK vs CTRL) FC||(FSK vs CTRL) LFC||Note||Link|
|1||218515_at||chromosome 21 open reading frame 66||NM_016631||94104||C21orf66||BM-020;GCFC||"gb:NM_016631.1 /DEF=Homo sapiens hypothetical protein (LOC51325), mRNA. /FEA=mRNA /GEN=LOC51325 /PROD=hypothetical protein /DB_XREF=gi:7706175 /UG=Hs.26461 hypothetical protein /FL=gb:AF208862.1 gb:NM_016631.1"||320.6||316.3||-1.01||-0.89||"all, "||HEK293T_Expression Human_ChIP_chip Human_CRE_prediction|
|2||221158_at||chromosome 21 open reading frame 66||NM_013329||94104||C21orf66||BM-020;GCFC||"gb:NM_013329.1 /DEF=Homo sapiens GC-rich sequence DNA-binding factor candidate (GCFC), mRNA. /FEA=mRNA /GEN=GCFC /PROD=GC-rich sequence DNA-binding factor candidate /DB_XREF=gi:7019390 /UG=Hs.283771 GC-rich sequence DNA-binding factor candidate /FL=gb:AF153208.1 gb:NM_013329.1"||884.01||809.76||-1.09||-0.95||"all, "||HEK293T_Expression Human_ChIP_chip Human_CRE_prediction|
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.
Guidelines for Choosing camp Induced Genes in this Data
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.