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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 211974_x_at recombining binding protein suppressor of hairless (Drosophila) AL513759966CD59 MIC11;MIN1;MIN2;MIN3;MSK21;PROTECTIN "Consensus includes gb:AL513759 /FEA=EST /DB_XREF=gi:12777253 /DB_XREF=est:AL513759 /CLONE=CL0BA008ZD02 (3 prime) /UG=Hs.278573 Homo sapiens similar to J KAPPA-RECOMBINATION SIGNAL BINDING PROTEIN (M. musculus) (LOC65437), mRNA" 1138.83 1334.51 1.17 1.09 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 200984_s_at "CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344)" X16447966CD59 MIC11;MIN1;MIN2;MIN3;MSK21;PROTECTIN "gb:X16447.1 /DB_XREF=gi:29805 /FEA=FLmRNA /CNT=428 /TID=Hs.119663.0 /TIER=Stack /STK=40 /UG=Hs.119663 /LL=966 /UG_GENE=CD59 /DEF=Human mRNA for CD59, an LY-6-like protein regulating complement membrane attack. /PROD=precursor polypeptide (AA -25 to 103) /FL=gb:NM_000611.1 gb:M34671.1" 3541.54 3332.87 -1.06 -0.99 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 200985_s_at "CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344)" NM_000611966CD59 MIC11;MIN1;MIN2;MIN3;MSK21;PROTECTIN "gb:NM_000611.1 /DB_XREF=gi:10835164 /GEN=CD59 /FEA=FLmRNA /CNT=428 /TID=Hs.119663.0 /TIER=FL+Stack /STK=60 /UG=Hs.119663 /LL=966 /DEF=Homo sapiens CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344) (CD59), mRNA. /PROD=CD59 antigen p18-20 (antigen identified bymonoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344) /FL=gb:NM_000611.1 gb:M34671.1" 3229.78 3035.04 -1.06 -0.99 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 200983_x_at "CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344)" BF983379966CD59 MIC11;MIN1;MIN2;MIN3;MSK21;PROTECTIN "gb:BF983379 /DB_XREF=gi:12386191 /DB_XREF=602305270F1 /CLONE=IMAGE:4396576 /FEA=FLmRNA /CNT=428 /TID=Hs.119663.0 /TIER=Stack /STK=83 /UG=Hs.119663 /LL=966 /UG_GENE=CD59 /UG_TITLE=CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344) /FL=gb:NM_000611.1 gb:M34671.1" 4349.61 4088.43 -1.06 -1.01 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.