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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 201025_at eukaryotic translation initiation factor 5B AB0182849669EIF5B DKFZp434I036;FLJ10524;IF2;KIAA0741 "gb:AB018284.1 /DB_XREF=gi:3882202 /GEN=KIAA0741 /FEA=FLmRNA /CNT=374 /TID=Hs.158688.0 /TIER=ConsEnd /STK=0 /UG=Hs.158688 /LL=9669 /DEF=Homo sapiens mRNA for KIAA0741 protein, complete cds. /PROD=KIAA0741 protein /FL=gb:AF078035.1 gb:AB018284.1 gb:NM_015904.1" 425.88 427.8 1 0.91 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 201027_s_at eukaryotic translation initiation factor 5B NM_0159049669EIF5B DKFZp434I036;FLJ10524;IF2;KIAA0741 "gb:NM_015904.1 /DB_XREF=gi:7706231 /GEN=IF2 /FEA=FLmRNA /CNT=374 /TID=Hs.158688.0 /TIER=FL /STK=0 /UG=Hs.158688 /LL=9669 /DEF=Homo sapiens KIAA0741 gene product (IF2), mRNA. /PROD=translation initiation factor IF2 /FL=gb:AF078035.1 gb:AB018284.1 gb:NM_015904.1" 363.65 372.79 1.03 0.9 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 214313_s_at Consensus includes gb:BE138647 /FEA=EST /DB_XREF=gi:8601147 /DB_XREF=est:xr78h03.x2 /CLONE=IMAGE:2766293 /UG=Hs.158688 KIAA0741 gene product BE1386479669EIF5B DKFZp434I036;FLJ10524;IF2;KIAA0741 Consensus includes gb:BE138647 /FEA=EST /DB_XREF=gi:8601147 /DB_XREF=est:xr78h03.x2 /CLONE=IMAGE:2766293 /UG=Hs.158688 KIAA0741 gene product 26.82 33.96 1.27 0.76 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 214314_s_at eukaryotic translation initiation factor 5B BE1386479669EIF5B DKFZp434I036;FLJ10524;IF2;KIAA0741 gb:BE138647 /DB_XREF=gi:8601147 /DB_XREF=xr78h03.x2 /CLONE=IMAGE:2766293 /FEA=EST /CNT=34 /TID=Hs.158688.1 /TIER=ConsEnd /STK=0 /UG=Hs.158688 /LL=9669 /UG_GENE=IF2 /UG_TITLE=KIAA0741 gene product 589.16 554.2 -1.06 -0.91 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
5 201024_x_at eukaryotic translation initiation factor 5B NM_0159049669EIF5B DKFZp434I036;FLJ10524;IF2;KIAA0741 Consensus includes gb:BG261322 /FEA=EST /DB_XREF=gi:12771138 /DB_XREF=est:602373079F1 /CLONE=IMAGE:4484563 /UG=Hs.158688 KIAA0741 gene product /FL=gb:AB018284.1 gb:AF078035.1 gb:NM_015904.1 948.83 912.05 -1.04 -0.94 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
6 201026_at "gb:AB018284.1 /DB_XREF=gi:3882202 /GEN=KIAA0741 /FEA=FLmRNA /CNT=374 /TID=Hs.158688.0 /TIER=ConsEnd /STK=0 /UG=Hs.158688 /LL=9669 /DEF=Homo sapiens mRNA for KIAA0741 protein, complete cds. /PROD=KIAA0741 protein /FL=gb:AF078035.1 gb:AB018284.1 gb:NM_015904.1" AB0182849669EIF5B DKFZp434I036;FLJ10524;IF2;KIAA0741 "gb:AB018284.1 /DB_XREF=gi:3882202 /GEN=KIAA0741 /FEA=FLmRNA /CNT=374 /TID=Hs.158688.0 /TIER=ConsEnd /STK=0 /UG=Hs.158688 /LL=9669 /DEF=Homo sapiens mRNA for KIAA0741 protein, complete cds. /PROD=KIAA0741 protein /FL=gb:AF078035.1 gb:AB018284.1 gb:NM_015904.1" 220.22 199.66 -1.1 -0.94 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.