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No probe set Gene Name Accession LocusLink Symbol Alias Symbols Description CTRL FSK (FSK vs CTRL) FC (FSK vs CTRL) LFC Note Link
1 214499_s_at BCL2-associated transcription factor 1 AF2492739774BCLAF1 BTF;KIAA0164 "gb:AF249273.1 /DB_XREF=gi:7582385 /FEA=FLmRNA /CNT=5 /TID=Hs.80338.1 /TIER=FL /STK=0 /UG=Hs.80338 /LL=9774 /UG_GENE=KIAA0164 /DEF=Homo sapiens Bcl-2-associated transcription factor short form mRNA, complete cds. /PROD=Bcl-2-associated transcription factor shortform /FL=gb:AF249273.1" 525.87 439.39 -1.2 -0.99 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
2 201083_s_at BCL2-associated transcription factor 1 AA7407549774BCLAF1 BTF;KIAA0164 gb:AA740754 /DB_XREF=gi:2779346 /DB_XREF=nz03b05.s1 /CLONE=IMAGE:1286673 /FEA=FLmRNA /CNT=305 /TID=Hs.80338.0 /TIER=Stack /STK=11 /UG=Hs.80338 /LL=9774 /UG_GENE=KIAA0164 /UG_TITLE=KIAA0164 gene product /FL=gb:NM_014739.1 gb:D79986.1 346.69 315.41 -1.1 -1.01 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
3 201101_s_at BCL2-associated transcription factor 1 BE9633709774BCLAF1 BTF;KIAA0164 gb:BE963370 /DB_XREF=gi:11766788 /DB_XREF=601657132R1 /CLONE=IMAGE:3866335 /FEA=FLmRNA /CNT=305 /TID=Hs.80338.0 /TIER=Stack /STK=9 /UG=Hs.80338 /LL=9774 /UG_GENE=KIAA0164 /UG_TITLE=KIAA0164 gene product /FL=gb:NM_014739.1 gb:D79986.1 787.72 655.87 -1.2 -1.11 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction
4 201084_s_at BCL2-associated transcription factor 1 NM_0147399774BCLAF1 BTF;KIAA0164 "gb:NM_014739.1 /DB_XREF=gi:7661957 /GEN=KIAA0164 /FEA=FLmRNA /CNT=305 /TID=Hs.80338.0 /TIER=FL+Stack /STK=69 /UG=Hs.80338 /LL=9774 /DEF=Homo sapiens KIAA0164 gene product (KIAA0164), mRNA. /PROD=KIAA0164 gene product /FL=gb:NM_014739.1 gb:D79986.1" 1216.46 1005.37 -1.21 -1.15 "all, " HEK293T_Expression Human_ChIP_chip Human_CRE_prediction

Sample Description:
Primary culture of human pancreatic islet was treated with cAMP agonist forskolin (FSK) and compared with control untreated samples. RNA was extracted using the Qiagen RNeasy kit. Two control and two forskolin treated samples were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133A 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing camp Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK with LFC>=1.3, we consider it as a good camp induced gene and therefore a potential CREB target.