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No probe set Symbol Alias Symbols Gene Name Accession LocusLink Description Fasting Refed Hepatocyte 0hr Hepatocyte 1hr FSK Hepatocyte 4hr FSK (Fasting vs Refed) FC (Fasting vs Refed) LFC (Hepatocyte 1hr FSK/0hr) FC (Hepatocyte 1hr FSK/0hr) LFC (Hepatocyte 4hr FSK/0hr) FC (Hepatocyte 4hr FSK/0hr) LFC Link
1 1438133_a_at Cyr61 CCN1;Igfbp10 cysteine rich protein 61 BM20277016007gb:BM202770 /DB_XREF=gi:17757100 /DB_XREF=C0244E04-3 /CLONE=C0244E04 /FEA=EST /CNT=12 /TID=Mm.1231.2 /TIER=Stack /STK=12 /UG=Mm.1231 /LL=16007 /UG_GENE=Cyr61 /UG_TITLE=cysteine rich protein 61 70.6 41.8 2751.1 1971.9 1881.4 1.69 0.00 -1.40 -1.30 -1.46 -1.37 "all, " MIN6_Expression Mouse_CRE_Prediction
2 1442340_x_at Cyr61 CCN1;Igfbp10 cysteine rich protein 61 BB53373616007"gb:BB533736 /DB_XREF=gi:9585665 /DB_XREF=BB533736 /CLONE=E030031K24 /FEA=EST /CNT=5 /TID=Mm.116967.1 /TIER=ConsEnd /STK=3 /UG=Mm.116967 /UG_TITLE=ESTs, Moderately similar to CYR6 MOUSE CYR61 PROTEIN PRECURSOR (M.musculus)" 95.8 99.7 449.2 319.7 392.5 -1.04 -0.25 -1.40 -1.25 -1.14 -1.01 "all, " MIN6_Expression Mouse_CRE_Prediction
3 1416039_x_at Cyr61 CCN1;Igfbp10 cysteine rich protein 61 NM_01051616007"gb:NM_010516.1 /DB_XREF=gi:6753593 /GEN=Cyr61 /FEA=FLmRNA /CNT=204 /TID=Mm.1231.1 /TIER=FL+Stack /STK=140 /UG=Mm.1231 /LL=16007 /DEF=Mus musculus cysteine rich protein 61 (Cyr61), mRNA. /PROD=cysteine rich protein 61 /FL=gb:M32490.1 gb:NM_010516.1" 1895.6 1896.7 3905.5 3402.5 2975.5 -1.00 -0.60 -1.15 -1.03 -1.31 -1.13 "all, " MIN6_Expression Mouse_CRE_Prediction
4 1457823_at Cyr61 CCN1;Igfbp10 cysteine rich protein 61 BB53373616007"gb:BB533736 /DB_XREF=gi:9585665 /DB_XREF=BB533736 /CLONE=E030031K24 /FEA=EST /CNT=5 /TID=Mm.116967.1 /TIER=ConsEnd /STK=3 /UG=Mm.116967 /UG_TITLE=ESTs, Moderately similar to CYR6 MOUSE CYR61 PROTEIN PRECURSOR (M.musculus)" 149.4 155.2 298.5 209.5 322.8 -1.04 -0.67 -1.43 -1.22 1.08 0.95 "all, " MIN6_Expression Mouse_CRE_Prediction

Sample Description:
Fasting/Refed: eight-week old male C57BL6/J mouse was fasted for 20 hr to induce glucagon-mediated cAMP signaling in hepatocytes (fasting sample). Control group (refed sample) was fasted for 16 hr then given food for 4 hr. Liver RNA was extracted using Trizol reagent and purified using the Qiagen RNeasy kit.
In parallel, mouse primary hepatocyte culture was treated with cAMP agonist forskolin (FSK) for 1hr or 4hr and total RNA was extracted using the Qiagen RNeasy kit.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Mouse Genome 430 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing cAMP Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by fasting (LFC>=1) in liver and was also induced by FSK in hepatocyte in at least one of the two time points (LFC>=1 at 1hr or 4hr), then we consider it as a good cAMP induced gene in liver and therefore a potential CREB target.