Search results are listed below.
Tip: To
quickly search the same gene in another data set, click the links from the last
column. The locuslink number will be used as query.
Sample Description:
Fasting/Refed: eight-week old male C57BL6/J mouse was fasted for 20 hr to induce
glucagon-mediated cAMP signaling in hepatocytes (fasting sample). Control group
(refed sample) was fasted for 16 hr then given food for 4 hr. Liver RNA was
extracted using Trizol reagent and purified using the Qiagen RNeasy kit.
In parallel, mouse primary hepatocyte culture was treated with cAMP agonist
forskolin (FSK) for 1hr or 4hr and total RNA was extracted using the Qiagen
RNeasy kit.
Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix
GeneChip Mouse Genome 430 2.0 Array. Array data was analyzed using the dChip
software with PM-MM models.
Guidelines for Choosing cAMP Induced Genes in this Data
Set:
The fold change (FC) value is positive for increased expression and negative
for decrease expression. There is also a lower bound of fold change (LFC), which
is a conservative estimation of the fold change with 90% confidence. For this
data set, if a gene was induced by fasting (LFC>=1) in liver and was also
induced by FSK in hepatocyte in at least one of the two time points (LFC>=1
at 1hr or 4hr), then we consider it as a good cAMP induced gene in liver and
therefore a potential CREB target.