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No probe set Symbol Alias Symbols Gene Name Accession LocusLink Description Fasting Refed Hepatocyte 0hr Hepatocyte 1hr FSK Hepatocyte 4hr FSK (Fasting vs Refed) FC (Fasting vs Refed) LFC (Hepatocyte 1hr FSK/0hr) FC (Hepatocyte 1hr FSK/0hr) LFC (Hepatocyte 4hr FSK/0hr) FC (Hepatocyte 4hr FSK/0hr) LFC Link
1 1456860_at Cdc26 2010012C09Rik RIKEN cDNA 2010012C09 gene BB76135666440"gb:BB761356 /DB_XREF=gi:16207093 /DB_XREF=BB761356 /CLONE=G270140H03 /FEA=EST /CNT=7 /TID=Mm.94715.1 /TIER=ConsEnd /STK=7 /UG=Mm.94715 /UG_TITLE=ESTs, Moderately similar to S12207 hypothetical protein (M.musculus)" 1600.5 1222.6 1166.8 1422.3 1258.6 1.31 1.23 1.22 1.14 1.08 1.01 "all, " MIN6_Expression Mouse_CRE_Prediction
2 1423987_at Cdc26 2010012C09Rik RIKEN cDNA 2010012C09 gene BC00577566440"gb:BC005775.1 /DB_XREF=gi:13543217 /FEA=FLmRNA /CNT=111 /TID=Mm.22192.1 /TIER=FL+Stack /STK=69 /UG=Mm.22192 /DEF=Mus musculus, clone MGC:12007 IMAGE:3602527, mRNA, complete cds. /PROD=Unknown (protein for MGC:12007) /FL=gb:BC005775.1" 688.0 729.5 596.1 564.2 464.5 -1.06 -0.94 -1.06 -0.87 -1.28 -1.14 "all, " MIN6_Expression Mouse_CRE_Prediction
3 1459540_at Cdc26 2010012C09Rik RIKEN cDNA 2010012C09 gene BB52828766440gb:BB528287 /DB_XREF=gi:16444629 /DB_XREF=BB528287 /CLONE=D930046A11 /FEA=EST /CNT=2 /TID=Mm.136484.1 /TIER=ConsEnd /STK=2 /UG=Mm.136484 /UG_TITLE=ESTs 3080.0 3084.7 3026.9 3270.6 2476.5 -1.00 -0.99 1.08 1.07 -1.22 -1.21 "all, " MIN6_Expression Mouse_CRE_Prediction
4 1452992_at Cdc26 2010012C09Rik RIKEN cDNA 2010012C09 gene AK00819966440"gb:AK008199.1 /DB_XREF=gi:12842240 /FEA=mRNA /CNT=27 /TID=Mm.193039.1 /TIER=Stack /STK=14 /UG=Mm.193039 /LL=66440 /UG_GENE=2010012C09Rik /UG_TITLE=RIKEN cDNA 2010012C09 gene /DEF=Mus musculus adult male small intestine cDNA, RIKEN full-length enriched library, clone:2010012C09:hypothetical protein, full insert sequence." 144.3 190.8 210.7 197.2 148.4 -1.32 -1.16 -1.07 -0.95 -1.42 -1.21 "all, " MIN6_Expression Mouse_CRE_Prediction

Sample Description:
Fasting/Refed: eight-week old male C57BL6/J mouse was fasted for 20 hr to induce glucagon-mediated cAMP signaling in hepatocytes (fasting sample). Control group (refed sample) was fasted for 16 hr then given food for 4 hr. Liver RNA was extracted using Trizol reagent and purified using the Qiagen RNeasy kit.
In parallel, mouse primary hepatocyte culture was treated with cAMP agonist forskolin (FSK) for 1hr or 4hr and total RNA was extracted using the Qiagen RNeasy kit.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Mouse Genome 430 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing cAMP Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by fasting (LFC>=1) in liver and was also induced by FSK in hepatocyte in at least one of the two time points (LFC>=1 at 1hr or 4hr), then we consider it as a good cAMP induced gene in liver and therefore a potential CREB target.