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No probe set Symbol Alias Symbols Gene Name Accession LocusLink Description Fasting Refed Hepatocyte 0hr Hepatocyte 1hr FSK Hepatocyte 4hr FSK (Fasting vs Refed) FC (Fasting vs Refed) LFC (Hepatocyte 1hr FSK/0hr) FC (Hepatocyte 1hr FSK/0hr) LFC (Hepatocyte 4hr FSK/0hr) FC (Hepatocyte 4hr FSK/0hr) LFC Link
1 1435261_at 1200009B18Rik 4930572C01Rik RIKEN cDNA 1200009B18 gene BQ17444267456"gb:BQ174442 /DB_XREF=gi:20349933 /DB_XREF=UI-M-DJ2-bvy-i-07-0-UI.s1 /CLONE=UI-M-DJ2-bvy-i-07-0-UI /FEA=EST /CNT=40 /TID=Mm.23956.1 /TIER=Stack /STK=18 /UG=Mm.23956 /UG_TITLE=ESTs, Weakly similar to F32D1.3.p (Caenorhabditis elegans) (C.elegans)" 331.9 222.9 278.5 206.7 314.1 1.49 1.15 -1.35 -1.10 1.13 0.92 "all, " MIN6_Expression Mouse_CRE_Prediction
2 1424462_at 1200009B18Rik 4930572C01Rik RIKEN cDNA 1200009B18 gene AK00466167456"gb:AK004661.1 /DB_XREF=gi:12835991 /FEA=FLmRNA /CNT=211 /TID=Mm.22179.1 /TIER=Stack /STK=28 /UG=Mm.22179 /LL=67456 /UG_GENE=1200009B18Rik /UG_TITLE=RIKEN cDNA 1200009B18 gene /DEF=Mus musculus adult male lung cDNA, RIKEN full-length enriched library, clone:1200009B18:homolog to CDA14, full insert sequence. /FL=gb:BC006895.1 gb:BC018188.1 gb:BC026558.1" 422.4 411.9 391.9 357.5 497.4 1.03 0.90 -1.10 -0.97 1.27 1.09 "all, " MIN6_Expression Mouse_CRE_Prediction
3 1439855_at 1200009B18Rik 4930572C01Rik RIKEN cDNA 1200009B18 gene AV34144967456"gb:AV341449 /DB_XREF=gi:16396949 /DB_XREF=AV341449 /CLONE=6430521H08 /FEA=EST /CNT=9 /TID=Mm.137858.1 /TIER=ConsEnd /STK=6 /UG=Mm.137858 /UG_TITLE=ESTs, Weakly similar to F32D1.3.p (Caenorhabditis elegans) (C.elegans)" 197.6 192.4 213.9 197.8 167.1 1.03 0.82 -1.08 -0.94 -1.28 -1.02 "all, " MIN6_Expression Mouse_CRE_Prediction
4 1451360_at 1200009B18Rik 4930572C01Rik RIKEN cDNA 1200009B18 gene BI43790867456gb:BI437908 /DB_XREF=gi:15262598 /DB_XREF=ic81d09.x1 /CLONE=IMAGE:5660200 /FEA=FLmRNA /CNT=211 /TID=Mm.22179.1 /TIER=ConsEnd /STK=5 /UG=Mm.22179 /LL=67456 /UG_GENE=1200009B18Rik /UG_TITLE=RIKEN cDNA 1200009B18 gene /FL=gb:BC006895.1 gb:BC018188.1 gb:BC026558.1 -674.0 -264.1 411.8 411.4 268.8 1.00 0.00 -1.00 -0.87 -1.53 -1.32 "all, " MIN6_Expression Mouse_CRE_Prediction
5 1453796_a_at 1200009B18Rik 4930572C01Rik RIKEN cDNA 1200009B18 gene AK01627567456"gb:AK016275.1 /DB_XREF=gi:12854943 /FEA=mRNA /CNT=7 /TID=Mm.22179.2 /TIER=ConsEnd /STK=0 /UG=Mm.22179 /LL=67456 /UG_GENE=1200009B18Rik /UG_TITLE=RIKEN cDNA 1200009B18 gene /DEF=Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone:4930572C01:homolog to CD002 PROTEIN, full insert sequence." 314.5 346.8 496.5 471.8 526.0 -1.10 -0.94 -1.05 -0.98 1.06 0.98 "all, " MIN6_Expression Mouse_CRE_Prediction

Sample Description:
Fasting/Refed: eight-week old male C57BL6/J mouse was fasted for 20 hr to induce glucagon-mediated cAMP signaling in hepatocytes (fasting sample). Control group (refed sample) was fasted for 16 hr then given food for 4 hr. Liver RNA was extracted using Trizol reagent and purified using the Qiagen RNeasy kit.
In parallel, mouse primary hepatocyte culture was treated with cAMP agonist forskolin (FSK) for 1hr or 4hr and total RNA was extracted using the Qiagen RNeasy kit.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Mouse Genome 430 2.0 Array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing cAMP Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by fasting (LFC>=1) in liver and was also induced by FSK in hepatocyte in at least one of the two time points (LFC>=1 at 1hr or 4hr), then we consider it as a good cAMP induced gene in liver and therefore a potential CREB target.