Search results are listed below.
Tip: To
quickly search the same gene in another data set, click the links from the last
column. The locuslink number will be used as query.
| No | probe set | Symbol | Alias Symbols | Gene Name | Accession | LocusLink | Description | Fasting | Refed | Hepatocyte 0hr | Hepatocyte 1hr FSK | Hepatocyte 4hr FSK | (Fasting vs Refed) FC | (Fasting vs Refed) LFC | (Hepatocyte 1hr FSK/0hr) FC | (Hepatocyte 1hr FSK/0hr) LFC | (Hepatocyte 4hr FSK/0hr) FC | (Hepatocyte 4hr FSK/0hr) LFC | Link | |
| 1 | 1423710_at | Dlst | 1600017E01Rik;4632413C10Rik;4930529O08Rik;DLTS | dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) | BC006702 | 78920 | "gb:BC006702.1 /DB_XREF=gi:13879445 /FEA=FLmRNA /CNT=219 /TID=Mm.28365.1 /TIER=FL+Stack /STK=141 /UG=Mm.28365 /LL=78920 /UG_GENE=4930529O08Rik /DEF=Mus musculus, RIKEN cDNA 4930529O08 gene, clone MGC:7935 IMAGE:3583936, mRNA, complete cds. /PROD=RIKEN cDNA 4930529O08 gene /FL=gb:BC006702.1" | 1033.5 | 939.1 | 936.9 | 959.7 | 820.4 | 1.10 | 0.94 | 1.02 | 0.89 | -1.14 | -1.02 | "all, " | MIN6_Expression Mouse_CRE_Prediction |
| 2 | 1437775_at | Dlst | 1600017E01Rik;4632413C10Rik;4930529O08Rik;DLTS | dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) | BB343361 | 78920 | gb:BB343361 /DB_XREF=gi:16404783 /DB_XREF=BB343361 /CLONE=B930044G13 /FEA=EST /CNT=18 /TID=Mm.133233.1 /TIER=Stack /STK=14 /UG=Mm.133233 /UG_TITLE=ESTs | 914.4 | 795.0 | 490.0 | 476.0 | 484.8 | 1.15 | 0.93 | -1.03 | -0.68 | -1.01 | -0.67 | "all, " | MIN6_Expression Mouse_CRE_Prediction |
| 3 | 1431011_at | Dlst | 1600017E01Rik;4632413C10Rik;4930529O08Rik;DLTS | dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) | AK019713 | 78920 | "gb:AK019713.1 /DB_XREF=gi:12860037 /FEA=mRNA /CNT=17 /TID=Mm.28365.2 /TIER=ConsEnd /STK=0 /UG=Mm.28365 /LL=78920 /UG_GENE=4930529O08Rik /UG_TITLE=RIKEN cDNA 4930529O08 gene /DEF=Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone:4930529O08:homolog to DIHYDROLIPOAMIDE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL PRECURSOR (EC 2.3.1.61) (E2) (E2K), full ins..." | 438.0 | 442.4 | 679.1 | 662.2 | 671.1 | -1.01 | -0.81 | -1.03 | -0.95 | -1.01 | -0.90 | "all, " | MIN6_Expression Mouse_CRE_Prediction |
| 4 | 1430161_at | Dlst | 1600017E01Rik;4632413C10Rik;4930529O08Rik;DLTS | dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) | AK005477 | 78920 | "gb:AK005477.1 /DB_XREF=gi:12838061 /FEA=mRNA /CNT=9 /TID=Mm.135216.1 /TIER=ConsEnd /STK=3 /UG=Mm.135216 /LL=74226 /UG_GENE=1600017E01Rik /UG_TITLE=RIKEN cDNA 1600017E01 gene /DEF=Mus musculus adult female placenta cDNA, RIKEN full-length enriched library, clone:1600017E01:homolog to DIHYDROLIPOAMIDE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL PRECURSOR (EC 2.3.1.61) (E2) (E2K), full..." | 1116.1 | 1251.3 | 1228.3 | 1200.8 | 940.4 | -1.12 | -1.08 | -1.02 | -0.99 | -1.31 | -1.27 | "all, " | MIN6_Expression Mouse_CRE_Prediction |
Sample Description:
Fasting/Refed: eight-week old male C57BL6/J mouse was fasted for 20 hr to induce
glucagon-mediated cAMP signaling in hepatocytes (fasting sample). Control group
(refed sample) was fasted for 16 hr then given food for 4 hr. Liver RNA was
extracted using Trizol reagent and purified using the Qiagen RNeasy kit.
In parallel, mouse primary hepatocyte culture was treated with cAMP agonist
forskolin (FSK) for 1hr or 4hr and total RNA was extracted using the Qiagen
RNeasy kit.
Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix
GeneChip Mouse Genome 430 2.0 Array. Array data was analyzed using the dChip
software with PM-MM models.
Guidelines for Choosing cAMP Induced Genes in this Data
Set:
The fold change (FC) value is positive for increased expression and negative
for decrease expression. There is also a lower bound of fold change (LFC), which
is a conservative estimation of the fold change with 90% confidence. For this
data set, if a gene was induced by fasting (LFC>=1) in liver and was also
induced by FSK in hepatocyte in at least one of the two time points (LFC>=1
at 1hr or 4hr), then we consider it as a good cAMP induced gene in liver and
therefore a potential CREB target.