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To quickly search the same gene in another data set, click the links from the last column. The locuslink number will be used as query.


No probe set Symbol gene Accession LocusLink Description GFP FSK_GFP (FSK_GFP vs GFP) FC (FSK_GFP vs GFP) LFC FSK_ACREB (FSK_ACREB vs FSK_GFP) FC (FSK_ACREB vs FSK_GFP) LFC ACREB (ACREB vs GFP) FC (ACREB vs GFP) LFC Link
1 97272_at Hnrpa1 heterogeneous nuclear ribonucleoprotein A1 M9916715382"Cluster Incl M99167:Mouse rearranged RNA binding protein (FLI-2) mRNA, complete cds /cds=(59,1021) /gb=M99167 /gi=193323 /ug=Mm.27927 /len=1443" 116.6 141.78 1.22 0.87 147.83 1.04 0.82 159.99 1.37 1.11 "all, " Liver_Expression Mouse_CRE_Prediction
2 92724_at Hnrpa1 heterogeneous nuclear ribonucleoprotein A1 AI18320215382"Cluster Incl AI183202:uc50h11.r1 Mus musculus cDNA, 5 end /clone=IMAGE-1429125 /clone_end=5 /gb=AI183202 /gi=3733840 /ug=Mm.46512 /len=314" 230.63 240.77 1.04 0.84 211.64 -1.14 -1.04 271.59 1.18 0.82 "all, " Liver_Expression Mouse_CRE_Prediction

Sample Description:
Culture of mouse pancreatic beta cell line MIN6 cells were infected with GFP or ACREB adenovirus, and treated with cAMP agonist forskolin (FSK) for 1hr. RNA was extracted using the Qiagen RNeasy kit. Two controls and two forskolin treated samples from GFP or ACREB virus infected cells were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Murine Genome U74 Version 2 Set MG-U74A array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing cAMP Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK in GFP infected samples (LFC>=1) and this induction was inhibited by ACREB ((FSK_ACREB vs FSK_GFP) LFC<=-1), we consider it as a good cAMP induced gene and therefore a potential CREB target.