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To quickly search the same gene in another data set, click the links from the last column. The locuslink number will be used as query.


No probe set Symbol gene Accession LocusLink Description GFP FSK_GFP (FSK_GFP vs GFP) FC (FSK_GFP vs GFP) LFC FSK_ACREB (FSK_ACREB vs FSK_GFP) FC (FSK_ACREB vs FSK_GFP) LFC ACREB (ACREB vs GFP) FC (ACREB vs GFP) LFC Link
1 96575_at Rpl8 ribosomal protein L8 U6777126961"Cluster Incl U67771:Mus musculus ribosomal protein L8 (RPL8) mRNA, complete cds /cds=(21,794) /gb=U67771 /gi=1527177 /ug=Mm.30066 /len=842" 3543.22 3873.2 1.09 0.98 3642.51 -1.06 -0.97 3670.05 1.04 0.93 "all, " Liver_Expression Mouse_CRE_Prediction
2 162476_r_at Rpl8 ribosomal protein L8 AV09284026961"Cluster Incl AV092840:AV092840 Mus musculus cDNA /clone=2310075N19 /clone_end=3 /gb=AV092840 /gi=5240388 /ug=Mm.53289 /len=290 /NOTE=replacement for probe set(s) 103164_i_at, 103165_f_at on MG-U74A" 435.46 432.83 -1.01 -0.8 374.45 -1.16 -1.06 410.71 -1.06 -0.84 "all, " Liver_Expression Mouse_CRE_Prediction

Sample Description:
Culture of mouse pancreatic beta cell line MIN6 cells were infected with GFP or ACREB adenovirus, and treated with cAMP agonist forskolin (FSK) for 1hr. RNA was extracted using the Qiagen RNeasy kit. Two controls and two forskolin treated samples from GFP or ACREB virus infected cells were analyzed.

Microarray Analysis:
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Murine Genome U74 Version 2 Set MG-U74A array. Array data was analyzed using the dChip software with PM-MM models.

Guidelines for Choosing cAMP Induced Genes in this Data Set:
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK in GFP infected samples (LFC>=1) and this induction was inhibited by ACREB ((FSK_ACREB vs FSK_GFP) LFC<=-1), we consider it as a good cAMP induced gene and therefore a potential CREB target.